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> Methicillin (sodium salt) [132-92-3]

Methicillin (sodium salt) [132-92-3]

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Cat# HY-B0974-1ml

Size : 10mM/1mL

Brand : MedChemExpress

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Description

Methicillin sodium salt (Meticillin sodium) is a β-lactam, semi-synthetic antibiotic related to penicillin antibiotic. Methicillin sodium salt inhibits penicillin-binding proteins involved in the synthesis of peptidoglycan. Methicillin sodium salt inhibits S. aureus with a MIC value of 2.1 μg/mL. Methicillin sodium salt can be used for the research of inflammation[1][2].

IC50 & Target

β-lactam

 

In Vitro

Methicillin sodium salt (100 μg/mL; 18 h) kills S. aureus after incubation intracellular for 18 hours[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methicillin sodium salt Related Antibodies

Cell Viability Assay[1]

Cell Line: Leukocytes
Concentration: 100 μg/mL
Incubation Time: 18 hours
Result: Effectively eliminated S. aureus with minimal bactericidal concentration of 3.13 μg/mL, and exhibited better effects under aerobical condition.
In Vivo

Methicillin sodium salt (42.5 and 85 mg/kg; i.m. four times daily; for 21 days or till spontaneous death) against the enterococcus in leukocytes[1].
Methicillin sodium salt (400 mg/kg; i.p. once) survives infected mice better than compared group[2].
Methicillin sodium salt (400 mg/kg; i.h. once) prevents infected mice from death[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: White New Zealand rabbits, weighing 2.0 to 3.0 kg[1]
Dosage: 42.5 and 85 mg/kg
Administration: Intramuscular injection; 42.5 and 85 mg/kg four times daily; for 21 days or till spontaneous death
Result: Inhibited enterococcus at 24 hours with a half-live of 1.1 h, but showed no statistical significance to rabbits.
Animal Model: Mice with 5-7 X 108 S. aureus[2]
Dosage: 400 mg/kg
Administration: Intraperitoneal injection; 400 mg/kg; once
Result: Showed a survival rate of 30.5%, higher than compared group with a survival rate of 6.9%.
Animal Model: Mice with 5-7 X 108 S. aureus[2]
Dosage: 400 mg/kg
Administration: Subcutaneous injection; 400 mg/kg; once
Result: Suppressed large abscesses developed in mice, and also prevented mice from death.
Clinical Trial
Molecular Weight

402.40

Synonyms

Meticillin sodium

Formula

C17H19N2NaO6S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C([C@H](N1C2=O)C(C)(S[C@@]1([C@@H]2NC(C3=C(C=CC=C3OC)OC)=O)12)C)O[Na]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (310.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (248.51 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4851 mL 12.4254 mL 24.8509 mL
5 mM 0.4970 mL 2.4851 mL 4.9702 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.17 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (5.17 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (248.51 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

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