Product Description  

miRNA Universal SYBR qPCR Master Mix is a special premix for qPCR assays based on SYBR Green I chimeric fluorescence method. The  quantification of miRNA requires high specificity because the miRNA sequences are short and miRNA sequences of the same family are generally highly similar. This product contains hot-start AceTaq DNA Polymerase based on the chemical modification. With the optimized Buffer, it can greatly reduce nonspecific amplification; meanwhile, the special ROX Reference Dye makes the master mix suitable for most qPCR instruments. It only needs to add primers and templates to perform amplification reaction, without adjusting the ROX concentration on different instruments. It is recommended to use with Vazyme's miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (Vazyme #MR101).

 Features  

• Specificity: Chemically modified Hot-start AceTaq DNA Polymerase, completely blocks the enzyme activity below 95°C, and is equipped with the specificity-enhancing factor, which has stronger amplification specificity.

• Superior reaction system: The optimized Buffer composition and concentration are more suitable for the qPCR amplification of microRNA.

• Broad qPCR instrument compatibility: The special ROX in the mix can be used on almost all qPCR instruments without adjusting the concentration.

• Software support: Provide online primer design software 'miRNA Primer Design'.

 Components  

 Storage  

Store at -30 ~ -15°C and protect from light. Transport at ≤0°C.