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> ATPase, p97

ATPase, p97

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Cat# A4001-10-500ul

Size : 500ul

Brand : US Biological


A4001-10 ATPase, p97

Clone Type
Monoclonal
Host
mouse
Source
Xenopus
Isotype
IgG1
Grade
Supernatant
Applications
WB
Crossreactivity
Hu Rb Rt Xe
Shipping Temp
Blue Ice
Storage Temp
-20°C

p97 ATPase is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p.

Applications:
Suitable for use in Western Blot. Not suitable for use in Immunofluorescence and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000. Dilution Buffer: 0.15M PBS, 0.1% BSA, 0.1% sodium azide (ECL: 5% milk powder in TBST)
Optimal dilutions to be determined by the researcher.

Positive Control:
Protein extracts from all vertebrate tissues and cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 5F125 (58.13.3)|Host: mouse|Source: Xenopus|Concentration: Not Determined|Form: Supplied as a liquid, 0.09% sodium azide.|Purity: Supernatant|Immunogen: 15S Mg2+ ATPase complex purified from Xenopus laevis ovary.|Specificity: Recognizes Xenopus ATPase, p97. Reacts with p97 subunit of 15S Mg2+-ATPase. Species Crossreactivity: rabbit, rat, human, vertebrate cells and tissues. Does not crossreact with the corresponding protein in budding yeast (Cdc48p). ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
15S Mg2+ ATPase complex purified from Xenopus laevis ovary.
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Supernatant
Specificity
Recognizes Xenopus ATPase, p97. Reacts with p97 subunit of 15S Mg2+-ATPase. Species Crossreactivity: rabbit, rat, human, vertebrate cells and tissues. Does not crossreact with the corresponding protein in budding yeast (Cdc48p).
References
1. Peters JM, Walsh MJ , and Franke WW: An abundant and ubiquitous homo-oligomeric ring-shaped ATPase particle related to the putative vesicle fusion proteins Sec18p and NSF. Embo J 9:1757-1767 (1990) 2. Peters JM, Harris JR, Lustig A, Müller S, Engel A, Volker S, and Franke WW: Ubiquitous soluble Mg2+ ATPase complex: a structural study. J Mol Biol 223:557-571 (1992) 3. Acharya U, Jacobs R, Peters JM, Watson N, Farquhar MG, and Malhotra V: The formation of Golgi stacks from vesiculated Golgi Membranes requires two distinct fusion events. Cell 82:895-904 (1995) 4. Rabouille C, TP Levine, JM Peters, and G Warren: An NSF-like ATPase, p97. and NSF mediate cisternal growth from mitotic Golgi fragments. Cell 82:905-915 (1995)