Reagents and instruments for immunology, cell biology and molecular biology.

mouse Anti-IgM, Fc (PE) Monoclonal Antibody [Clone:CH2]

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Cat# 052434-100ug

Size : 100ug

Brand : US Biological

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052434 IgM, Fc (PE)

Clone Type

Monoclonal

Host

mouse

Source

human

Isotype

IgG1

Grade

Highly Purified

Applications

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Immunoglobulin M (IgM) is produced as a 900kD pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12g/l and its biological survival (plasma T1/2) is 5 days.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1-4ug/ml. Extracellular and intracellular staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: CH2|Host: mouse|Source: human|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). |Purity: Purified by size-exclusion chromatography.|Immunogen: Purified human IgM.|Specificity: Recognizes human IgM, Fc-region localized epitope.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Purified human IgM.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).

Purity

Purified by size-exclusion chromatography.

Specificity

Recognizes human IgM, Fc-region localized epitope.

References

1. Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.|2. Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.|3. Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.|4 Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.