RayBio® G-Series Mouse Cytokine Antibody Array 3 Kit. Detects 62 Mouse Cytokines. Suitable for all liquid sample types.
Mouse Cytokine Array G3
Mouse Cytokine Array G3
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 62 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Axl
| BLC (CXCL13)
| CD30 Ligand (TNFSF8)
| CD30 (TNFRSF8)
| CD40 (TNFRSF5)
|
| CRG-2
| CTACK (CCL27)
| CXCL16
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
| Fas Ligand (TNFSF6)
| Fractalkine (CX3CL1)
| GCSF
| GM-CSF
| IFN-gamma
|
| IGFBP-3
| IGFBP-5
| IGFBP-6
| IL-1 beta (IL-1 F2)
| IL-10
|
| IL-12 p40/p70
| IL-12 p70
| IL-13
| IL-17A
| IL-1 alpha (IL-1 F1)
|
| IL-2
| IL-3
| IL-3 R beta
| IL-4
| IL-5
|
| IL-6
| IL-9
| KC (CXCL1)
| Leptin
| Leptin R
|
| LIX
| L-Selectin (CD62L)
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
| MCP-5
|
| M-CSF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 gamma
| MIP-2
|
| MIP-3 beta (CCL19)
| MIP-3 alpha (CCL20)
| Platelet Factor 4 (CXCL4)
| P-Selectin
| RANTES (CCL5)
|
| SCF
| SDF-1 alpha (CXCL12 alpha)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| TARC (CCL17)
|
| I-309 (TCA-3/CCL1)
| TECK (CCL25)
| TIMP-1
| TNF alpha
| Thrombopoietin (TPO)
|
| VCAM-1 (CD106)
| VEGF-A
| |||
Species Detected
Mouse
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array G3 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAM-CYT-G3-4.gal)
GAL (GAL-AAM-CYT-G3-8.gal)
Excel (AAM-CYT-G3_Analysis_Tool.xls)
citations
METTL1 promotes tumorigenesis through tRNA-derived fragment biogenesis in prostate cancer
- Species:
- Mouse
- Sample Type:
- prostate tissue
- Year:
- 2023
- Journal Name:
- Molecular Cancer
Deletion of NoxO1 limits atherosclerosis development in female mice
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2020
- Journal Name:
- Redox biology
PTH Signaling in Osteoprogenitors Is Essential for B-Lymphocyte Differentiation and Mobilization
- Species:
- Mouse
- Sample Type:
- Bone Marrow Aspirate (Mone marrow supernates from control and Osx-PPR KO mice)
- Year:
- 2015
- Journal Name:
- Journal of Bone and Mineral Research
- PubMed ID:
- 26191777
Safety and efficacy of composite collagen-silver nanoparticle hydrogels as tissue engineering scaffolds
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Subcutaneous tissue around an implanted hydrogel in lower back.)
- Year:
- 2015
- Journal Name:
- Nanoscale
- PubMed ID:
- 26507748
CD73 on B16F10 melanoma cells in CD73-deficient mice promotes tumor growth, angiogenesis, neovascularization, macrophage infiltration and metastasis
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (B16F10 melanoma model)
- Year:
- 2015
- Journal Name:
- International Journal of Biochemistry and Cell Biology
- PubMed ID:
- 26545615
Anti-Atherosclerotic and Anti-Inflammatory Actions of Sesame Oil
- Species:
- Mouse
- Sample Type:
- Plasma (Seasame oil treated mice)
- Year:
- 2015
- Journal Name:
- Journal of Medicinal Food
- PubMed ID:
- 25562618
Anti-inflammatory peptides from cardiac progenitors ameliorate dysfunction after myocardial infarction
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Cardiac progenitor cells, Skeletal myobalstas, and adipose-derived mesenchymal cells)
- Year:
- 2014
- Journal Name:
- Journal of the American Heart Association
- PubMed ID:
- 25468657
Platelets guide the formation of early metastatic niches
- Species:
- Mouse
- Sample Type:
- Conditioned Media (platelet releasates)
- Year:
- 2014
- Journal Name:
- PNAS
Macrophage subpopulations are essential for infarct repair with and without stem cell therapy.
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (myocardium lysate - effect of MSCs on macrophage polarization and function after myocardial infarct)
- Year:
- 2013
- Journal Name:
- Journal of The American College of Cardiology
- PubMed ID:
- 23973704
Hyperlipidemia Impaired Innate Immune Response to Periodontal Pathogen Porphyromonas gingivalis in ApolipoproteinE Knockout Mice
- Species:
- Mouse
- Sample Type:
- Peritoneal Fluid (Peritoneal Fluids)
- Year:
- 2013
- Journal Name:
- Plos One