RayBio® Mouse Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Mouse Leptin ELISA Kit
Mouse Leptin ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Sensitivity | 4 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 4 pg/ml - 1000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF- 1alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 10 - 40 fold |
| Accession Number | P41160 |
| Gene Id | 16846 |
| Gene Symbols | LEP, OB |
| Protein Name / Synonyms | Leptin (Obesity factor) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Autophagy, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Mouse Leptin ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Leptin (Lenep), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Maternal probiotic supplementation protects against PBDE-induced developmental, behavior and metabolic reprogramming in a sexually dimorphic manner: Role of gut microbiome
- Species:
- Mouse
- Sample Type:
- Plasma (adult offspring, ad libitum fed)
- Year:
- 2025
- Journal Name:
- Archives of Toxicology
- PubMed ID:
- 39520540
Chronic pharmacologic manipulation of dopamine transmission ameliorates metabolic disturbance in Trappc9-linked brain developmental syndrome
- Species:
- Mouse
- Sample Type:
- Plasma (Trappc9-KO and WT mice)
- Year:
- 2024
- Journal Name:
- JCI Insight
- PubMed ID:
- 38889014
Moderate-intensity aerobic exercise attenuates obesity-induced changes in perivascular adipose tissue in female mice
- Species:
- Mouse
- Sample Type:
- Serum (obese female mice, exercise)
- Year:
- 2025
- Journal Name:
- Physiological Reports
- PubMed ID:
- 40842420
Thermogenic adipocytes alleviate hepatic steatosis and insulin resistance via macrophage cytokine secretion in obese mice
- Species:
- Mouse
- Sample Type:
- Plasma (high-fat-diet obese mice)
- Year:
- 2025
- Journal Name:
- bioRxiv
Leptin/obR signaling exacerbates obesity-related neutrophilic airway inflammation through inflammatory M1 macrophages
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Molecular Medicine
1α,25-Dihydroxyvitamin D Downregulates Adipocyte Impact on Breast Cancer Cell Migration and Adipokine Release
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (3T3-L1 adipocytes, 1,25 OH2D treatment)
- Year:
- 2024
- Journal Name:
- Nutrients
- PubMed ID:
- 39339753
Metabolic dysfunction in mice with adipocyte-specific ablation of the adenosine A2A receptor
- Species:
- Mouse
- Sample Type:
- Serum (A2AR-FKO mice, 12-week HFD)
- Year:
- 2025
- Journal Name:
- Journal of Biological Chemistry
- PubMed ID:
- 39828097
Transient Receptor Potential (TRP) based polypharmacological combination stimulates energy expending phenotype to reverse HFD-induced obesity in mice
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- LIFE SCIENCES
Sema7A protects against high-fat diet-induced obesity and hepatic steatosis by regulating adipo/lipogenesis
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Molecular metabolism
Differential curcumin absorption and curcumin-induced STAT3 inhibition during 3T3-L1 cell adipogenesis in 2D and 3D cultures
- Species:
- Mouse
- Sample Type:
- Other / Unspecified (media from differentiated 3T3-L1 adipocytes)
- Year:
- 2024
- Journal Name:
- Discover Applied Sciences
