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Mouse Monoclonal Anti-CD68 (Macrophage Marker) [Clone KP1]
Cat# NB-36-01020-P0
Size : 20ug
Brand : Neo Biotech
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Phone : +1 850 650 7790
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Printable Datasheet
CD68 (Macrophage Marker) Antibody [KP1]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Tonsil stained with CD68 Mouse Monoclonal Antibody (KP1).
SDS-PAGE Analysis of Purified CD68 Mouse Monoclonal Antibody (KP1). Confirmation of Purity and Integrity of Antibody.
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Cat, Human, Monkey, Mouse, Rabbit, Rat
Research Areas
Immunology, Hematopoietic Stem Cells
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Endosome membrane, Lysosome membrane
Gene Name
CD68
Positive Control
lymph node or spleen., Tonsil
Immunogen
Subcellular fraction of human alveolar macrophages
Alternate Names
Macrosialin, Gp110, GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1
Functions
- Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Key References
- Pulford KA et. al. Journal of Clinical Pathology, 1989, 42(4):414-21.
- Warnke RA et. al. Am J of Pathol, 1989, 135:1089-95.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/7680921/
- https://pubmed.ncbi.nlm.nih.gov/14702039/
- https://pubmed.ncbi.nlm.nih.gov/16625196/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/9790779/
- https://pubmed.ncbi.nlm.nih.gov/15194571/
- https://pubmed.ncbi.nlm.nih.gov/15647451/
- https://pubmed.ncbi.nlm.nih.gov/16263699/
- https://pubmed.ncbi.nlm.nih.gov/18405323/
- https://pubmed.ncbi.nlm.nih.gov/19159218/
- https://pubmed.ncbi.nlm.nih.gov/25944712/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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