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Mouse Monoclonal Anti-MUC1 / EMA / CD227 (Epithelial Marker) [Clone MUC1/955]
Cat# NB-36-00519-P1
Size : 100ug
Brand : Neo Biotech
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Phone : +1 850 650 7790
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Printable Datasheet
MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody [MUC1/955]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (MUC1/955).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (MUC1/955).
Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (MUC1/955).
SDS-PAGE Analysis Purified MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (MUC1/955).
Western Blot Analysis of human T47D cell lysate using MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (MUC1/955).
Western Blot Analysis of human MCF-7 cell lysate using MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (MUC1/955).
Applications & Dilutions
Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol
Summary
This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human, Mouse
Research Areas
Immunology, Cytokine Signaling, Infectious Disease
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cytoplasm, Nucleus, Secreted
Gene Name
MUC1
Positive Control
Colon, endometrial carcinoma., MCF-7 or MDA-231 cells. Breast, Ovarian
Immunogen
Human milk-fat globule membranes (HMFGM)
Alternate Names
Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen
Functions
- The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
- The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
Key References
- Stanley CM, Phillips TE. Am J Physiol. 1999;277:G191-200.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2394722/
- https://pubmed.ncbi.nlm.nih.gov/2318825/
- https://pubmed.ncbi.nlm.nih.gov/1697589/
- https://pubmed.ncbi.nlm.nih.gov/2268309/
- https://pubmed.ncbi.nlm.nih.gov/2351132/
- https://pubmed.ncbi.nlm.nih.gov/2112460/
- https://pubmed.ncbi.nlm.nih.gov/1688329/
- https://pubmed.ncbi.nlm.nih.gov/7925397/
- https://pubmed.ncbi.nlm.nih.gov/9212228/
- https://pubmed.ncbi.nlm.nih.gov/15623537/
- https://pubmed.ncbi.nlm.nih.gov/22941036/
- https://pubmed.ncbi.nlm.nih.gov/15969018/
- https://pubmed.ncbi.nlm.nih.gov/16710414/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/3417635/
- https://pubmed.ncbi.nlm.nih.gov/2597151/
- https://pubmed.ncbi.nlm.nih.gov/8608966/
- https://pubmed.ncbi.nlm.nih.gov/8604237/
- https://pubmed.ncbi.nlm.nih.gov/11341784/
- https://pubmed.ncbi.nlm.nih.gov/15987679/
- https://pubmed.ncbi.nlm.nih.gov/10373415/
- https://pubmed.ncbi.nlm.nih.gov/7988707/
- https://pubmed.ncbi.nlm.nih.gov/7664271/
- https://pubmed.ncbi.nlm.nih.gov/7744025/
- https://pubmed.ncbi.nlm.nih.gov/9139698/
- https://pubmed.ncbi.nlm.nih.gov/9312074/
- https://pubmed.ncbi.nlm.nih.gov/9536947/
- https://pubmed.ncbi.nlm.nih.gov/9597769/
- https://pubmed.ncbi.nlm.nih.gov/9819408/
- https://pubmed.ncbi.nlm.nih.gov/10197628/
- https://pubmed.ncbi.nlm.nih.gov/11173916/
- https://pubmed.ncbi.nlm.nih.gov/11118479/
- https://pubmed.ncbi.nlm.nih.gov/11152665/
- https://pubmed.ncbi.nlm.nih.gov/11350974/
- https://pubmed.ncbi.nlm.nih.gov/11483589/
- https://pubmed.ncbi.nlm.nih.gov/11877440/
- https://pubmed.ncbi.nlm.nih.gov/14521915/
- https://pubmed.ncbi.nlm.nih.gov/12750561/
- https://pubmed.ncbi.nlm.nih.gov/14688481/
- https://pubmed.ncbi.nlm.nih.gov/12441351/
- https://pubmed.ncbi.nlm.nih.gov/12832415/
- https://pubmed.ncbi.nlm.nih.gov/12939402/
- https://pubmed.ncbi.nlm.nih.gov/15471854/
- https://pubmed.ncbi.nlm.nih.gov/15710329/
- https://pubmed.ncbi.nlm.nih.gov/16288032/
- https://pubmed.ncbi.nlm.nih.gov/15972891/
- https://pubmed.ncbi.nlm.nih.gov/15513966/
- https://pubmed.ncbi.nlm.nih.gov/16507569/
- https://pubmed.ncbi.nlm.nih.gov/16427018/
- https://pubmed.ncbi.nlm.nih.gov/17524503/
- https://pubmed.ncbi.nlm.nih.gov/17308127/
- https://pubmed.ncbi.nlm.nih.gov/17545600/
- https://pubmed.ncbi.nlm.nih.gov/16983337/
- https://pubmed.ncbi.nlm.nih.gov/20816948/
- https://pubmed.ncbi.nlm.nih.gov/23186163/
- https://pubmed.ncbi.nlm.nih.gov/23396133/
- https://pubmed.ncbi.nlm.nih.gov/16585136/
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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