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Mouse Monoclonal Anti-MUC1 / EMA / CD227 (Epithelial Marker) [Clone MUC1/845]
Cat# NB-36-00525-P0
Size : 20ug
Brand : Neo Biotech
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Phone : +1 850 650 7790
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Printable Datasheet
MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody [MUC1/845]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC-1 Mouse Monoclonal Antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC-1 Mouse Monoclonal Antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 Mouse Monoclonal Antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with MUC-1 Mouse Monoclonal Antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 Mouse Monoclonal Antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 Mouse Monoclonal Antibody (MUC1/845).
SDS-PAGE Analysis Purified MUC-1 Mouse Monoclonal Antibody (MUC1/845). Confirmation of Integrity and Purity of Antibody.
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions.In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cancer, Immunology, Cytokine Signaling, Infectious Disease
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cytoplasm, Nucleus, Secreted
Gene Name
MUC1
Positive Control
Colon, endometrial carcinoma., MCF-7 or MDA-231 cells. Breast, Ovarian
Immunogen
Human milk-fat globule membranes (HMFGM)
Alternate Names
Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
Functions
- The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
- The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
Key References
- Rughetti, A., et al. 2005. Recombinant tumor-associated Mucin 1 glycoprotein impairs the differentiation and function of dendritic cells. J. Immunol. 174: 7764-7772.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2394722/
- https://pubmed.ncbi.nlm.nih.gov/2318825/
- https://pubmed.ncbi.nlm.nih.gov/1697589/
- https://pubmed.ncbi.nlm.nih.gov/2268309/
- https://pubmed.ncbi.nlm.nih.gov/2351132/
- https://pubmed.ncbi.nlm.nih.gov/2112460/
- https://pubmed.ncbi.nlm.nih.gov/1688329/
- https://pubmed.ncbi.nlm.nih.gov/7925397/
- https://pubmed.ncbi.nlm.nih.gov/9212228/
- https://pubmed.ncbi.nlm.nih.gov/15623537/
- https://pubmed.ncbi.nlm.nih.gov/22941036/
- https://pubmed.ncbi.nlm.nih.gov/15969018/
- https://pubmed.ncbi.nlm.nih.gov/16710414/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/3417635/
- https://pubmed.ncbi.nlm.nih.gov/2597151/
- https://pubmed.ncbi.nlm.nih.gov/8608966/
- https://pubmed.ncbi.nlm.nih.gov/8604237/
- https://pubmed.ncbi.nlm.nih.gov/11341784/
- https://pubmed.ncbi.nlm.nih.gov/15987679/
- https://pubmed.ncbi.nlm.nih.gov/10373415/
- https://pubmed.ncbi.nlm.nih.gov/7988707/
- https://pubmed.ncbi.nlm.nih.gov/7664271/
- https://pubmed.ncbi.nlm.nih.gov/7744025/
- https://pubmed.ncbi.nlm.nih.gov/9139698/
- https://pubmed.ncbi.nlm.nih.gov/9312074/
- https://pubmed.ncbi.nlm.nih.gov/9536947/
- https://pubmed.ncbi.nlm.nih.gov/9597769/
- https://pubmed.ncbi.nlm.nih.gov/9819408/
- https://pubmed.ncbi.nlm.nih.gov/10197628/
- https://pubmed.ncbi.nlm.nih.gov/11173916/
- https://pubmed.ncbi.nlm.nih.gov/11118479/
- https://pubmed.ncbi.nlm.nih.gov/11152665/
- https://pubmed.ncbi.nlm.nih.gov/11350974/
- https://pubmed.ncbi.nlm.nih.gov/11483589/
- https://pubmed.ncbi.nlm.nih.gov/11877440/
- https://pubmed.ncbi.nlm.nih.gov/14521915/
- https://pubmed.ncbi.nlm.nih.gov/12750561/
- https://pubmed.ncbi.nlm.nih.gov/14688481/
- https://pubmed.ncbi.nlm.nih.gov/12441351/
- https://pubmed.ncbi.nlm.nih.gov/12832415/
- https://pubmed.ncbi.nlm.nih.gov/12939402/
- https://pubmed.ncbi.nlm.nih.gov/15471854/
- https://pubmed.ncbi.nlm.nih.gov/15710329/
- https://pubmed.ncbi.nlm.nih.gov/16288032/
- https://pubmed.ncbi.nlm.nih.gov/15972891/
- https://pubmed.ncbi.nlm.nih.gov/15513966/
- https://pubmed.ncbi.nlm.nih.gov/16507569/
- https://pubmed.ncbi.nlm.nih.gov/16427018/
- https://pubmed.ncbi.nlm.nih.gov/17524503/
- https://pubmed.ncbi.nlm.nih.gov/17308127/
- https://pubmed.ncbi.nlm.nih.gov/17545600/
- https://pubmed.ncbi.nlm.nih.gov/16983337/
- https://pubmed.ncbi.nlm.nih.gov/20816948/
- https://pubmed.ncbi.nlm.nih.gov/23186163/
- https://pubmed.ncbi.nlm.nih.gov/23396133/
- https://pubmed.ncbi.nlm.nih.gov/16585136/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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