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Mouse Monoclonal Anti-MUC6 (Mucin 6 / Gastric Mucin) [Clone SPM598]
Cat# NB-36-00539X-P1
Size : 100ug
Brand : Neo Biotech
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Phone : +1 850 650 7790
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Printable Datasheet
MUC6 (Mucin 6 / Gastric Mucin) Antibody [SPM598]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC6 Monoclonal Antibody (SPM598).
Applications & Dilutions
Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
The MUC6 gastric mucin is a secreted glycoprotein that plays an essential role in epithelial cyto-protection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract. Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileum and right colon. In gastric cancer, Mucin 6 has an altered expression. In normal stomach, Mucin 6 is associated with Lewis type 2; Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas. Mucin 6 is a secretory mucin, located in the deeper mucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Immunology, Infectious Disease
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Secreted
Gene Name
MUC6
Positive Control
Human stomach tissue (IHC).
Immunogen
A synthetic peptide of the Gastric Mucin 6 tandem repeat sequence
Alternate Names
Mucin-6, Gastric mucin-6, Gastric mucin 6; MUC6; MUC6 mucin; Mucin 6 oligomeric mucus/gel forming; Mucin glycoprotein Fragment; Mucin-6; Secretory mucin MUC6
Functions
- May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Plays an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis.
Key References
- Reis C A, David L, Correa P, et al. Intestinal metaplasia of human stomach displays distinct patterns of mucin (MUC1, MUC2, MUC5AC, and MUC6) expression. Cancer Res. 59(5): 1003-1007 (1999)
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/16554811/
- https://pubmed.ncbi.nlm.nih.gov/15081123/
- https://pubmed.ncbi.nlm.nih.gov/7680650/
- https://pubmed.ncbi.nlm.nih.gov/14702039/
- https://pubmed.ncbi.nlm.nih.gov/9195947/
- https://pubmed.ncbi.nlm.nih.gov/9422745/
- https://pubmed.ncbi.nlm.nih.gov/10209489/
- https://pubmed.ncbi.nlm.nih.gov/10330458/
- https://pubmed.ncbi.nlm.nih.gov/11988092/
- https://pubmed.ncbi.nlm.nih.gov/15280409/
- https://pubmed.ncbi.nlm.nih.gov/15979574/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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