N-Glycolylneuraminic acid

Biological Activity

N-Glycolylneuraminic acid is a nonhuman sialic acid molecule synthesized in pigs but not in humans. N-Glycolylneuraminic acid works as a decoy receptor of N-Glycolylneuraminic acid-binding influenza A viruses (IAVs).

Chemical Information

Molecular Weight	325.27
Formula	C11H19NO10
CAS Number	1113-83-3
Solubility (25°C)	Water ≥ 60 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Cindy M Spruit, et al. J Virol. N-Glycolylneuraminic Acid Binding of Avian and Equine H7 Influenza A Viruses

[2] Renato Mastrangeli, et al. Trends Pharmacol Sci. Current views on N-glycolylneuraminic acid in therapeutic recombinant proteins

[3] Sharon Yehuda, et al. Front Immunol. Glycosylated Biotherapeutics: Immunological Effects of N-Glycolylneuraminic Acid

[4] Andrea Perota, et al. Front Immunol. N-Glycolylneuraminic Acid (Neu5Gc) Null Large Animals by Targeting the CMP-Neu5Gc Hydroxylase (CMAH)

[5] Anoopjit Singh Kooner, et al. Front Immunol. Synthesis of N-Glycolylneuraminic Acid (Neu5Gc) and Its Glycosides