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> Pannexin-2 Antibody

Pannexin-2 Antibody

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Cat# ASM10254-A700-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - Pannexin-2 Antibody ASM10254
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Pannexin-2 Monoclonal Antibody, Clone S121A-31 (ASM10254). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Pannexin-2 Monoclonal Antibody (ASM10254) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X.
  • WB - Pannexin-2 Antibody ASM10254
    Western Blot analysis of Rat Brain Membrane showing detection of ~70 kDa Pannexin 2 protein using Mouse Anti-Pannexin 2 Monoclonal Antibody, Clone S121A-31 (ASM10254). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Pannexin 2 Monoclonal Antibody (ASM10254) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~70 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC
Primary Accession P60571
Other Accession NP_955441.2
Host Mouse
Isotype IgG1
Reactivity Human, Rat
Clonality Monoclonal
Description Mouse Anti-Rat Pannexin-2 Monoclonal IgG1
Target/Specificity Detects ~70kDa. Does not cross-react with Pannexin-1 or Pannexin-3.
Other Names Pannexin 2 Antibody, PANX2 Antibody, PX2 Antibody, hPANX2 Antibody, MGC119432 Antibody
Clone Names S121A-31
Immunogen Fusion protein amino acids 11-664 (all but first 10 amino acids, encompassing extracellular and cytoplasmic domains) of rat Pannexin-2
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-420 was sufficient for detection of Pannexin-2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Cell Junction | Gap Junction