PDPN Rat Monoclonal Antibody [Clone ID: 8F11]
Specifications
Product Data | |
Clone Name | 8F11 |
Applications | FC, IHC, IP, WB |
Recommended Dilution | Western blot: 1 μg/ml for chemiluminescence detection system. For details see protocols below. Immunoprecipitation: 1-2 μg/250 μl of cell extract from 2.5x106 cells. Immunohistochemistry on Paraffin Sections: 1.25 μg/ml (whole mount). Flow Cytometry: 5-10 μg/ml (final concentration). Immunocytochemistry: It is reported that this antibody can be used in immunocytochemistry (see reference no. 2) |
Reactivities | Mouse |
Host | Rat |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | NL-17 cell membrane fraction |
Specificity | This antibody reacts with Mouse Aggrus (Podoplanin). The reference Kato, Y., et al. (2003) described that this antibody does not react to Human Aggrus (Podoplanin). |
Formulation | PBS containing 50% glycerol, pH 7.2 State: Azide Free State: Liquid Ig fraction Preservative: None |
Concentration | lot specific |
Purification | Protein G agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store (in aliqouts) at -20 °C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Predicted Protein Size | 44 kDa |
Gene Name | podoplanin |
Database Link | |
Background | In addition to hemostasis and host defense, platelets are involved in the induction of inflammation, tissue repair, and tumor metastasis. Aggrus (T1a/Podoplanin) is a novel 36-45 kDa membrane sialoglycoprotein that induces platelet aggregation. Aggrus is highly expressed in various tumor cells and the platelet aggregation ability of Aggrus depends on specific oligosaccharide structures that may be related to the metastasis process in cancer cells. Aggrus has been identified as a diagnostic tumor marker for seminomas and testicular cancers. |
Synonyms | Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025 |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting |
Reference Data |
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