Reagents and instruments for immunology, cell biology and molecular biology.

Peroxidase from horseradish min. 1 000 U/mg

Print

Cat# 31941.02

Size : 10MG

Contact local distributor :

Phone : +1 850 650 7790

Brand : SERVA Electrophoresis

Contact local distributor :

Phone : +1 850 650 7790

(POD, HRP, Donor: hydrogen-peroxide oxidoreductase)
EC 1.11.1.7 • M_r ca. 40 000 • CAS [9003-99-0]

For the determination of peroxide (1). Used as an indicator enzyme in reactions where peroxide is produced (2). For labelling antibodies in ELISA (3, 4). RZ (= A 403/A 275) = 3.0.

Unit definition: 1 U catalyzes the decomposition of 1 µmole hydrogen peroxide per minute at 25 °C, pH 7.0; reaction coupled with phenol- aminoantipyrine (5).
Activity in other units: min. 250 purpurogallin units/mg
(1 purpurogallin unit catalyzes the oxidation of 1 mg pyrogallol to purpurogallin in 20 seconds at 20 °C and pH 6.0. The purpurogallin is extracted and determined spectrophotometrically at 420 nm (6). 1 mg purpurogallin requires 13.5 µmole peroxide, 1 purpurogallin unit corresponds to the decomposition of 12 µmoles peroxide at 25 °C.)

References:

Meiattini, F. (1985) Methods of Enzymatic Analysis, (Bergmeyer, H.U., ed.) 3rd Ed. vol. 7, p. 566-71
Bergmeyer, H.U. (1983) Methods of Enzymatic Analysis, 3rd Ed. vol. 2, p. 267-8
Johnson Jr., R.B. (1980) J. Immunoassay 1, 27-37
Harlow & Lane (1988) Antibodies, Cold Spring Harbor Laboratory Press, p. 349
Gallati, H. (1977) J. Clin. Chem. Clin. Biochem. 15, 699-703
Polis, B.D. & Shmukler, H.W. (1963) J. Biol. Chem. 201, 457-500