Application
| WB, IHC-P, IF, ICC, FC |
|---|---|
| Primary Accession | Q01860 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 39kDa |
| Dilution | ICC (1:100-1:200), IF (1:100-1:200), IHC-P (10 µg/ml), WB (1:500-1:3000) , |
| Gene ID | 5460 |
|---|---|
| Other Names | POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3 |
| Target/Specificity | Human OCT3/4. Predicted cross-reactivity based on amino acid sequence homology: rabbit (85%), bovine (85%), pig (88%), monkey (97%). |
| Reconstitution & Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Precautions | POU5F1 / OCT4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POU5F1 |
|---|---|
| Synonyms | OCT3, OCT4, OTF3 |
| Function | Transcription factor that binds to the octamer motif (5'- ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Cellular Location | Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern. Colocalizes with MAPK8 and MAPK9 in the nucleus. {ECO:0000250|UniProtKB:P20263, ECO:0000269|PubMed:18191611, ECO:0000269|PubMed:19274063, ECO:0000269|PubMed:23024368} |
| Tissue Location | Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. |
| Volume | 50 µl |