rabbit Anti-KNG1 (Kininogen 1, BDK, KNG, HMWK, Alpha-2- Thiol Proteinase Inhibitor, Bradykinin, Kallidin, Fitzgerald factor, High molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, Ile-Ser-Bradykinin) Polyclonal Antibody

High molecular weight kininogen (HMWK) plays an important role in assembly of the plasma kallikrein-kinin system. The KNG1 gene generates both HMWK and low molecular weight kininogen (LMWK) through alternative splicing. Both HMWK and LMWK contain an identical heavy chain consisting of protein domains 1, 2, and 3. However, HMWK contains a 56kD light chain that consists of domains 5 and 6H, whereas LMWK contains a unique 4kD light chain that consists of domain 5L. In both proteins, the heavy and light chains are linked by domain 4, which contains the bradykinin (BK) nonapeptide. BK, which is released by plasma kallikrein, is a potent inflammatory mediator that causes vasodilation and enhanced capillary permeability, induces pain, and stimulates production of nitric oxide and prostacyclin from endothelial cells.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

AA Sequence:
MGHHHHHHSGS-EAQEIDCND EAVFQAVDFS LKQFNPGVKS GNQYMLHRVI EGTKTDGSPT FYSFKYLIKE GNCSAQSGLA WQDCDFKDAE EAATGECTAT VGKRENEFFI VTQTCKIAPS KAPILKAYFP CIGCVHAIST DSPDLEPVLK HSIEHFNNNT DHSHLFTLRK VKSAHRQVVA GLNFDITYTI VQTNCSKERF PSLHGDCVAL PNGDDGECRG NLFMDINNKI ANFSQSCTLY SGDDLVEALP KPCPGCPRDI PVDSPELKEV LGHSIAQLNA ENDHPFYYKI DTVKKATSQV VAGTKYVIEF IARETKCSKE SNTELAEDCE IKHLGQSLDC NANVYMRPWE NKVVPTVKCQ ALDMTEMAR

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.