Rabbit Interleukin 6,IL-6 ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

Brand : Cusabio

Cat# CSB-E06903Rb-24T

Reagents and instruments for immunology, cell biology and molecular biology.

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Size : 24T

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SDS

CSB-E06903Rb

Size	96T,5×96T,10×96T

Target Name

interleukin 6 (interferon, beta 2)

Associated names

IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit

Abbreviation

Uniprot No.

Species

Oryctolagus cuniculus (Rabbit)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-92
1:2	Average %	99
	Range %	91-105
1:4	Average %	101
	Range %	92-110
1:8	Average %	93
	Range %	86-98

Recovery

The recovery of rabbit IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	96	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.840	2.785	2.813	2.710
500	2.316	2.223	2.270	2.167
250	1.580	1.567	1.574	1.471
125	0.935	0.874	0.905	0.802
62.5	0.479	0.462	0.471	0.368
31.2	0.266	0.254	0.260	0.157
15.6	0.170	0.158	0.164	0.061
0	0.107	0.099	0.103

ELISA Data Analysis

Troubleshooting
and FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Description

This Rabbit IL6 ELISA Kit was designed for the quantitative measurement of Rabbit IL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Average Rating:

5.0 - 4 reviews

Sample type: Tissue homogenate(liver)

Sample species: Rabbit

Review: I tried liver and serum samples treated and untreated rabbits, the idea was to see with concentration was the best to perform real experiments. The kit works better using liver samples.

By Anonymous

Sample type: Plasma (anticoagulant)

Sample species: Rabbit

Sample dilution: No dilution

Review: I used CSB-E06903Rb to detect the plasma of the model group before operation, 30min-2 hours during operation, and 24 hours after operation. The R2 of the standard curve fitted by the four-parameter model was 0.99984 (see the picture for details). The standard curve met the expectation and...

By Anonymous

Sample type: Plasma (anticoagulant)

Sample species: Rabbit

Sample dilution: No dilution

Review: The second time, I used CSB-E06903Rb to detect the plasma of the treatment group before surgery, 30min-2 hours during surgery, and 24 hours after surgery. The R2 of the standard curve fitted by the four-parameter model was 0.99998 (see the picture), the standard curve was in line with the...

By Anonymous

Sample type: Plasma (anticoagulant)

Sample species: Rabbit

Sample dilution: No dilution

Review: I used CSB-E06903Rb to measure the plasma of the model group before operation, 30min-2 hours during operation, and 24 hours after operation. The R2 of the standard curve fitted by the four-parameter model was 0.99913 (see the picture for details). The standard curve was made in two columns,...

By Anonymous

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the...

Gene References into Functions

Mild heat shock increased the production of inflammatory cytokines, IL-1beta and IL-6 in rabbit cornea cells.

Subcellular Location

Secreted.

Protein Families

IL-6 superfamily

Database Links

KEGG:

STRING:

UniGene: