RAPSN, Recombinant, Mouse, aa1-412, His-Tag (Receptor Associated Protein of the Synapse, 43kD Receptor-Associated Protein of the Synapse, RING Finger Protein 205, RNF205)

43kD receptor-associated protein of the synapse, also known as RAPSN, is expressed in the postsynaptic membrane of skeletal muscle. RAPSN is required for the clustering of nicotinic acetylcholine receptors (nAChR). It self-associates through at least two of its seven tetra-tricopeptide repeats (TPRs). RAPSN interacts with the large intracellular domain of the nAChR a subunit through the hydrophobic surface of the coiled-coil domain. This protein modifies trafficking of AChR within the cell. Overexpression inhibits agrin-induced AChR clustering pathway.

Source:
Recombinant protein corresponding to aa1-412 from mouse RAPSN, fused to His-tag at N-terminus, expressed in E.coli.

Molecular Weight:
~48.8kD (435aa)

Applications:
Suitable for use in SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.