Quantibody® Rat Cytokine Array 67 Kit: A combination of 2 non-overlapping arrays (QAR-CYT-3 (27) & QAR-CYT-4 (40)) to quantitatively measure the concentration of 67 rat cytokines. Suitable for all liquid sample types.
Rat Cytokine Array Q67
Rat Cytokine Array Q67
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Rat |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 67 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Antibody Drug Development, Biomarker Detection |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 10 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| 4-1BB (TNFRSF9/CD137)
| Activin A
| Adiponectin (ACRP30)
| beta-NGF
| CD48 (SLAMF2)
|
| CD80 (B7-1)
| CD86 (B7-2)
| CINC-1 (CXCL1)
| CINC-2
| CINC-3
|
| CNTF
| CTACK (CCL27)
| Decorin
| Eotaxin-1 (CCL11)
| EphA5
|
| Erythropoietin
| FGF-BP
| Flt-3 Ligand
| Fractalkine (CX3CL1)
| Galectin-1
|
| Galectin-3
| Gas 1
| GFR alpha-1 (GDNF R)
| GM-CSF
| gp130
|
| HGF
| ICAM-1 (CD54)
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
| IL-1 R6 (IL-1 Rrp2)
| IL-1 Ra (IL-1 F3)
| IL-2
| IL-2 R alpha
| IL-3
|
| IL-4
| IL-6
| IL-7
| IL-10
| IL-13
|
| IL-17F
| IL-22
| JAM-A (CD321/F11R)
| LIX
| L-Selectin (CD62L)
|
| MCP-1 (CCL2)
| MIP-1 alpha (CCL3)
| Neuropilin-1
| Neuropilin-2
| Nope
|
| Notch-1
| Notch-2
| P-Cadherin
| PDGF-AA
| Prolactin
|
| Prolactin R
| RAGE
| RANTES (CCL5)
| SCF
| TCK-1 (CXCL7)
|
| TIM-1 (KIM-1)
| TIMP-1
| TIMP-2
| TNF alpha
| TREM-1
|
| TWEAK R (TNFRSF12)
| VEGF-A
| |||
Species Detected
Rat
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Rat Cytokine Array Q3 Slide(s); Rat Cytokine Array Q4 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktails
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAR-CYT-3.gal)
GAL (GAL-QAR-CYT-4.gal)
Excel (QAR-CYT-3_Analysis_Tool.xls)
Excel (QAR-CYT-4_Analysis_Tool.xls)
citations
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) alleviate excessive autophagy of ovarian granular cells through VEGFA/PI3K/AKT/mTOR pathway in premature ovarian failure rat model
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of ovarian research
The therapeutic effect of Shenhua tablet against mesangial cell proliferation and renal inflammation in mesangial proliferative glomerulonephritis
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Biomedicine & Pharmacotherapy
Effect of valproic acid upon skeletal muscle subjected to prolonged tourniquet application
- Species:
- Rat
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Trauma surgery & acute care open
CINC-2 and miR-199a-5p in EVs secreted by transplanted Thy1+ cells activate hepatocytic progenitor cell growth in rat liver regeneration
- Species:
- Rat
- Sample Type:
- mesenchymal cells (MCs)
- Year:
- 2023
- Journal Name:
- Stem Cell Research & Therapy
Pharmacological Characterization of a Novel Neuroactive Steroid Prodrug, NTS-104, and Its Neuroprotective Activity in Experimental Stroke Models
- Species:
- Rat
- Sample Type:
- cortex, plasma
- Year:
- 2022
- Journal Name:
- Stroke
Monocrotaline-Induced Pulmonary Arterial Hypertension and Bosentan Treatment in Rats: Focus on Plasma and Erythrocyte Parameters
- Species:
- Rat
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Pharmaceuticals
Gefitinib-Induced Cutaneous Toxicities in Brown Norway Rats Are Associated with Macrophage Infiltration
- Species:
- Rat
- Sample Type:
- Tissue Lysate (skin)
- Year:
- 2020
- Journal Name:
- Inflammation
Protein microarray analysis of cytokine expression changes in distal stumps after sciatic nerve transection.
- Species:
- Rat
- Sample Type:
- Tissue Lysate (extracted from distal nerve stumps of sciatic nerves)
- Year:
- 2020
- Journal Name:
- Neural Regeneratoin Research
Differential Regulation of Cardiac Function and Intracardiac Cytokines by Rapamycin in Healthy and Diabetic Rats
- Species:
- Rat
- Sample Type:
- Tissue Lysate (Heart Tissue lysate from diabetic rats)
- Year:
- 2017
- Journal Name:
- Oxidative Medicine and Cellular Longevity
- PubMed ID:
- 28408970
Inhibition of neuropathic hyperalgesia by intrathecal bone marrow stromal cells is associated with alteration of multiple soluble factors in cerebrospinal fluid
- Species:
- Rat
- Sample Type:
- CSF (CSF relief of pain when injected)
- Year:
- 2017
- Journal Name:
- Experimental Brain Research
- PubMed ID:
- 28573310