Orb776175-96Tests | Rat Transforming Growth Factor Beta 1 (TGFb1)

Rat Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

SKU: orb776175

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Description

The Rat Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit uses a sandwich enzyme immunoassay to quantify Transforming Growth Factor Beta 1 (TGFb1) in rat serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit has a detection range of 15.63-1000 pg/mL and a sensitivity of 6.2 pg/mL.

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Tested Applications	ELISA
Application Notes	standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	6.2 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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TGF Beta 1,TGF-B1 ELISA Kit, CED ELISA Kit, DPD1 ELISA Kit, LAP ELISA Kit, Camurati-Engelmann Disease ELISA Kit, Latency-associated peptide ELISA Kit

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Rat TGFb1(Transforming Growth Factor Beta 1) Microsample ELISA Kit [orb2998841]
Reactivity:
Rat
Dynamic Range:
15.63-1000 pg/mL
Sensitivity:
6.1 pg/mL
Sizes Available: 48 T, 96 T
Rat TGF Beta 1 ELISA Kit [orb50104]
Reactivity:
Rat
Dynamic Range:
15.6 pg/ml - 1,000 pg/ml
Sensitivity:
<1 pg/ml
Sizes Available: 96 T
Rat TGF-β1 (Transforming Growth Factor Beta 1) Quick ELISA Kit [orb2567541]
Reactivity:
Rat
Dynamic Range:
31.25-2000pg/ml
Sensitivity:
18.75pg/ml
Sizes Available: 48 T, 96 T
Rat PDGF-BB ELISA Kit [orb507539]
Reactivity:
Rat
Dynamic Range:
62.5 pg/ml - 4,000 pg/ml
Sensitivity:
<10 pg/ml
Sizes Available: 96 T
Rat TGF beta 1 ELISA Kit [orb411378]
Reactivity:
Rat
Dynamic Range:
6.25 pg/mL-400 pg/mL
Sensitivity:
1.56 pg/mL
Sizes Available: 48 T, 96 T, 5 x 96 T, 10 x 96 T, 24 T