SARS-CoV-2 N ELISA Kit
Cat# CSB-EL33251-10x96T
Size : 10x96T
Brand : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Novel Coronavirus Nucleoprotein (SARS-CoV-2 N)
Associated names
N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Abbreviation
SARS-CoV-2 N
Uniprot No.
Sample Types
serum, plasma, swabs
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Infectious Diseases
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||
| Inter-assay Precision (Precision between assays): CV%<10% | ||
| Three samples of known concentration were tested in twenty assays to assess. | ||
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean(ng/ml) | 25.39 | 24.46 | 26.31 | 26.65 | 26.31 | 24.19 |
| SD | 0.027 | 0.025 | 0.031 | 0.041 | 0.035 | 0.037 |
| CV(%) | 5.7 | 5.46 | 6.33 | 8.26 | 7.13 | 8.01 |
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| ng/ml | OD1 | OD2 | Average | Corrected |
| 200 | 3.228 | 3.104 | 3.166 | 3.032 |
| 100 | 2.045 | 2.169 | 2.107 | 1.973 |
| 50 | 1.001 | 0.931 | 0.966 | 0.832 |
| 25 | 0.469 | 0.507 | 0.488 | 0.354 |
| 12.5 | 0.268 | 0.276 | 0.272 | 0.138 |
| 6.25 | 0.196 | 0.204 | 0.200 | 0.066 |
| 3.12 | 0.168 | 0.161 | 0.165 | 0.031 |
| 0 | 0.136 | 0.132 | 0.134 |
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Citations
- S Zhou, Y Xu, H Liao, H Ou, D Qi, Y Wu, Y Liu, J Li, J Li,Genes & Diseases,2025
Customer Reviews and Q&A
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Target Background
Function
(From Uniprot)
(From Uniprot)
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Subcellular Location
Virion. Host cytoplasm.
Protein Families
Betacoronavirus nucleocapsid protein family