ASM10376-A565-100 | SOD (Cu/Zn) Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody (ASM10376). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody (ASM10376) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Cu/Zn) Antibody. (C) Composite.
Western blot analysis of Human Cell line lysates showing detection of SOD1 protein using Rabbit Anti-SOD1 Polyclonal Antibody (ASM10376). Load: 15 µg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-SOD1 Polyclonal Antibody (ASM10376) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody (ASM10376). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody (ASM10376) at 1:120 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Cu/Zn) Antibody. (C) Composite.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, IP, ICC
Primary Accession	P07632
Other Accession	NP_058746.1
Host	Rabbit
Reactivity	Human, Mouse, Rat, Bovine
Clonality	Polyclonal
Description	Rabbit Anti-Rat SOD (Cu/Zn) Polyclonal
Target/Specificity	Detects ~23kDa (human) and ~19kDa (other species).
Other Names	Superoxide dismutase1 Antibody, ALS1 Antibody, IPOA Antibody, SOD1 Antibody, SOD2 Antibody, SODC Antibody
Immunogen	Rat Cu/Zn SOD
Purification	Protein A Purified
Storage	-20ºC
Storage Buffer	PBS pH7.0, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	0.5 µg/ml of SPC-115 was sufficient for detection of Cu/Zn SOD in 20 µg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.
Cellular Localization	Cytoplasm

SOD (Cu/Zn) Antibody