TEV Protease Recombinant

TEV Protease Recombinant
Catalog number: B2014184
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 kU
Molecular Weight or Concentration: 27 kDa
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: P1 protease, TEVp, Tobacco Etch Virus protease, rTEV
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Tropea JE, Cherry S, Waugh DS. Expression and purification of soluble His(6)-tagged TEV protease Methods Mol Biol. 2009;498:297-307.
2: Raran-Kurussi S, Cherry S, Zhang D, Waugh DS. Removal of Affinity Tags with TEV Protease Methods Mol Biol. 2017;1586:221-230.
3: Sanchez MI, Ting AY. Directed evolution improves the catalytic efficiency of TEV protease Nat Methods. 2020 Feb;17(2):167-174.
4: Paththamperuma C, Page RC. Fluorescence dequenching assay for the activity of TEV protease Anal Biochem. 2022 Dec 15;659:114954.
5: Wang T, Yang N, Liang C, Xu H, An Y, Xiao S, Zheng M, Liu L, Wang G, Nie L. Detecting Protein-Protein Interaction Based on Protein Fragment Complementation Assay Curr Protein Pept Sci. 2020;21(6):598-610.
6: Kapust RB, Tzsr J, Copeland TD, Waugh DS. The P1′ specificity of tobacco etch virus protease Biochem Biophys Res Commun. 2002 Jun 28;294(5):949-55.
7: Rizza JD, Ortega C, Carri_n F, Fl_ M, Correa A. Production, purification and characterization of a double-tagged TEV protease Protein Expr Purif. 2022 Mar;191:106021.
8: Mohammadian N, Mohammadian H, Moazen F, Pakdel MH, Jahanian-Najafabadi A, Mir Mohammad Sadeghi H. Optimization of solvent media to solubilize TEV protease using response surface method Res Pharm Sci. 2020 Aug 28;15(4):331-339.
9: Renna P, Ripoli C, Dagliyan O, Pastore F, Rinaudo M, Re A, Paciello F, Grassi C. Engineering a switchable single-chain TEV protease to control protein maturation in living neurons Bioeng Transl Med. 2022 Feb 22;7(2):e10292.
10: Raran-Kurussi S, Waugh DS. Expression and Purification of Recombinant Proteins in Escherichia coli with a His(6) or Dual His(6)-MBP Tag Methods Mol Biol. 2017;1607:1-15.

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