ASM10223-STR-100 | Thorase/Atad1 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATAD1 ATPase Monoclonal Antibody, Clone S125-10 (ASM10223). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody (ASM10223) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and accumulates around nucleus.
Western Blot analysis of Rat brain membrane lysate showing detection of ATAD1 ATPase protein using Mouse Anti-ATAD1 ATPase Monoclonal Antibody, Clone S125-10 (ASM10223). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody (ASM10223) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Mouse Anti-ATAD1 ATPase Monoclonal Antibody, Clone S125-10 (ASM10223). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody (ASM10223) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining in all basal cells.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC/IF, AM
Primary Accession	Q9D5TO
Other Accession	NP_080763.2
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Mouse Thorase/Atad1 Monoclonal IgG1
Target/Specificity	Detects ~40kDa.
Other Names	ATAD1 antibody, AAA+ ATPase Thorase antibody, Ab2-088 antibody, AFDC1 antibody, ATAD1_HUMAN antibody, ATPase family AAA domain containing protein 1 antibody, ATPase family AAA domain-containing protein 1 antibody, ATPase family, AAA domain containing 1 antibody, ATPase Thorase antibody, AW107648 antibody, FLJ14600 antibody, FNP001 antibody, Thorase antibody
Clone Names	S125-10
Immunogen	Fusion protein amino acids 1-361 (Full length) of mouse Thorase
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-347 was sufficient for detection of Thorase in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Peroxisome

Thorase/Atad1 Antibody