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> TLR4 Antibody

TLR4 Antibody

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Cat# ASM10462-A565-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • IHC - TLR4 Antibody ASM10462
    Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462). Tissue: colon colitis. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Localization: Inflammatory cells.
  • WB - TLR4 Antibody ASM10462
    Western blot analysis of Human, Mouse Cell line lysates showing detection of TLR4 protein using Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462). Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462) at 1:1000.
  • IHC - TLR4 Antibody ASM10462
    Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462). Tissue: Spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462) at 1:100.
  • IHC - TLR4 Antibody ASM10462
    Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462). Tissue: spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462) at 1:100.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FACS, FCM, ICC/IF
Primary Accession O00206
Other Accession NP_612564.1
Host Rabbit
Reactivity Human, Mouse
Clonality Polyclonal
Description Rabbit Anti-Human TLR4 Polyclonal
Target/Specificity Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
Other Names ARMD10 Antibody, CD284 Antibody, TLR 4 Antibody, TOLL Antibody, Toll like receptor 4 Antibody
Immunogen Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Membrane