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> Trehalose 6,6′-dimycolate [61512-20-7]

Trehalose 6,6′-dimycolate [61512-20-7]

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Cat# HY-W145657-1mg

Size : 1mg

Brand : MedChemExpress


Description

Trehalose 6,6'-dimycolate (Cord Factor) is trehalose 6,6'-dimycolate, a cell wall glycolipid of Mycobacterium tuberculosis, which can be used to simulate inflammation and granuloma induced by Mycobacterium tuberculosis (MTB) form. Trehalose 6,6′-dimycolate also protects Mycobacterium tuberculosis from macrophage-mediated killing, inhibits efficient antigen presentation, and reduces the development of protective T cell responses[1][2].

In Vivo

1. Induce inflammation and granuloma formation[1]
Pathogenic mechanism
Trehalose 6,6’-dimycolate prevents calcium-mediated fusion of phagosomes and lysosomes, inhibits phagolysosome acidification, and thereby inhibits the killing effect of mouse macrophages on Mycobacterium tuberculosis, impairs the body's effective immune response, and induces the expression of matrix metalloproteinases.
Specific modeling method
Mice: C57BL/6 • female • 6-8 week-old (period: 7 days)
Administration: 0.5 or 1.25 mg/kg • i.v., or i.p. first then i.v. • once
Modeling success index
Phenotypic observation ：Macrophages gather locally, the size and number of granulomas increase, inflammation intensifies, alveolar spaces decrease, there are small hemorrhages, and lymphocytes mainly infiltrate at the junction of granulomas and blood vessels.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C57BL/6 mice (6-8 weeks, 20 g)[1]
Dosage: 0.5, 1.25 mg/kg (10 μg, 25 μg per 20 g mice)
Administration: Intravenous injection (i.v.) once (0.5, 1.25 mg/kg), or received 0.5 mg/kg via Intraperitoneal injection (i.p.) followed by an i.v. after 7 days of 0.5 mg/kg
Result: Increased the lung weight index (LWI). Local aggregation of macrophages was observed 7 days later at 0.5 mg/kg. The size and number of granulomas increased, inflammation intensified, alveolar spaces decreased, small hemorrhagic petechiae appeared, and lymphocytes mainly infiltrated at the intersection of granulomas and blood vessels at 1.25 mg/kg. In i.p.+i.v. model, the inflammatory response was more aggressive. Induced vascular remodeling and collagen deposition.
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Trehalose 6,6?dimycolate]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Purity & Documentation
References