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> GRP170 Antibody

GRP170 Antibody

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Cat# ASM10160-P594-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - GRP170 Antibody ASM10160
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6E3-2C3 (ASM10160). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody (ASM10160) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GRP170 Antibody (D) Composite.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q9Y4L1
Other Accession NP_006389.3
Host Mouse
Isotype IgG2b
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human GRP170 Monoclonal IgG2bK
Target/Specificity Detects ~170kDa.
Other Names ORP150 Antibody, HSP12A Antibody, Hypoxia up regulated 1 Antibody, Orp150 Antibody, Glucose regulated Antibody 170 Antibody, 150kDa oxygen regulated Antibody Antibody
Clone Names 6E3-2C3
Immunogen Raised against a synthetic peptide of human GRP170
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-232 was sufficient for detection of GRP170 in 20 µg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Endoplasmic Reticulum | Endoplasmic Reticulum Lumen