EpCAM antibody [VU-1D9] (PerCP-Cy5.5)
Cat# GTX00606-11-100test
Size : 100test
Brand : Genetex
Contact local distributor :
Phone : +1 850 650 7790
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | VU-1D9 |
Isotype | IgG1 |
Application | FACS |
Reactivity | Human |
| Package | 100 test |
Datasheet File View product citations for antibody GTX00606-11 on CiteAb
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| FACS | 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension |
Not tested in other applications.
Calculated MW
35 kDa. ( Note )
Product Note
This antibody recognizes an epitope within EGF-like domain I of CD326 / EpCAM.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Small cell lung carcinoma cell line H69.
Purification
Purified by size-exclusion chromatography
Conjugation
Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5)
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Cy and CyDye are trademarks of GE Healthcare.
TARGET
Synonyms
DIAR5 , EGP2 , EGP314 , EGP40 , EPCAM , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK1 , TACSTD1 , TROP1 , epithelial cell adhesion molecule , EpCAM , CD326
Cellular Localization
Lateral cell membrane,Cell junction,Tight junction
Background
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Database
Clone Reference
Alecia-Jane Twigger et al. Nat Commun 2022; 13 (1):562 Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk.
Hideharu Mikami et al. bioRxiv 2020; (Epub) Virtual-freezing fluorescence imaging flow cytometry.
Hideharu Mikami et al. Nat Commun 2020; 11 (1):1162 Virtual-freezing fluorescence imaging flow cytometry
Yoshida M et al. Biotechnol Bioeng 2017; (Epub) Preferential capture of EpCAM-expressing extracellular vesicles on solid surfaces coated with an aptamer-conjugated zwitterionic polymer.
Lyberopoulou A et al. PLoS One 2015; 10 (4):e0123902 Mutational analysis of circulating tumor cells from colorectal cancer patients and correlation with primary tumor tissue.
Submit a Reference REVIEW
Clone Reference
Alecia-Jane Twigger et al. Nat Commun 2022; 13 (1):562 Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk.
Hideharu Mikami et al. bioRxiv 2020; (Epub) Virtual-freezing fluorescence imaging flow cytometry.
Hideharu Mikami et al. Nat Commun 2020; 11 (1):1162 Virtual-freezing fluorescence imaging flow cytometry
Yoshida M et al. Biotechnol Bioeng 2017; (Epub) Preferential capture of EpCAM-expressing extracellular vesicles on solid surfaces coated with an aptamer-conjugated zwitterionic polymer.
Lyberopoulou A et al. PLoS One 2015; 10 (4):e0123902 Mutational analysis of circulating tumor cells from colorectal cancer patients and correlation with primary tumor tissue.
Stephen Kershaw et al. BMC Cancer 2015; 15:387 Optimisation of immunofluorescence methods to determine MCT1 and MCT4 expression in circulating tumour cells
Chen HH et al. J Biomed Opt 2014; 19 (4):45002 High-purity separation of cancer cells by optically induced dielectrophoresis.
| SDS | |
|---|---|
| PBS.pdf | |
| Sodium Azide.pdf | |