![ELISA](https://www.clinisciences.com/en/upload/thumbs/elisa-couleurs-pzb3e3ts-2qYF2Q5N-35e650.jpg)
ELISA
![pre-coated ELISA](https://www.clinisciences.com/en/upload/thumbs/270612_124336_PEEL_ceQYeU-d98dfc.jpg)
ELISA Kits
I. Required material
Reagents
- A primary antibody detection, specific to the target molecule
- A recombinante molecule to use as a standard
- Detection Solutions: e.g. Streptavidin-HRP and TMB
Buffers
- Blocking Buffer: e.g. PBS with 5%BSA
- Wash Buffer: e.g. PBS with 0.05%Tween
- Diluent for Sample and Standard: e.g. PBS 1%BSA
- Stop Solution: e.g. diluted H2SO4
Material - 96 well microtitre plates precoated with capture...
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![uncoated ELISA](https://www.clinisciences.com/en/upload/thumbs/270612_124319_PEEL_hGTGw8-da83c9.jpg)
I. Required material
Reagents
- A primary antibody capture, specific to the target molecule
- A second primary antibody detection, specific to the target molecule
- A recombinante molecule to use as a standard
- Detection Solutions: e.g. Streptavidin-HRP and TMB
Buffers
- Coating Buffer: e.g. PBS
- Blocking Buffer: e.g. PBS with 5%BSA
- Wash Buffer: e.g. PBS with 0.05%Tween
- Diluent for Sample and Standard: e.g. PBS 1%BSA
- Stop Solution: e...
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