Custom service : Chicken polyclonals production

Custom service : Chicken polyclonals production

Use of chickens allow the obtention of antibodies against highly conserved proteins from mammalians species by a technique involving egg yolk, an alternative way to the animal experiments.

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This prestation is realized from peptide, recombinant protein, cDNA (genetic immunization), small organisms, haptens, protein extracts etc…

Standard protocol :
- Peptide design
- Peptide synthesis ou Recombinant protein production
- KLH labeling (or any other carrier) if necessary
- Collection of pre-immune eggs
- Immunization of the chicken
- Collection of immune eggs
- Delivery of IgY prepared by ammonium sulphate precipitation of the egg yolk
- Option : Affinity purification against the antigen or IgY specific purification

Key numbers :
- Minimal number of animals in the protocol : 2
- Protocol length : 70 days
- Number of injections per animal : 4
- Quantity of antigen per injection : 100-250 µg
- Number of sample collections per animal : 0
- Final amount of antibodies per animal : 15-20 mg

We can develop polyclonal antibodies from other species : Rabbit, Guinea Pig, Rat, Mouse, Chinese Hamster, Goat, Donkey, Sheep.

Related services available :
=> Gene synthesis
=> Gene cloning
=> Peptide synthesis/Recombinant production
=> Other polyclonals production
=> Monoclonals production
=> Antibody labeling
=> Antibody purification
=> Antibody fragmentation
=> Antibody refolding
=> Antibody validation