Construction of basic vectors

Construction of basic vectors

Vectors built from standard backbones and standard components

Turnaround time for basic vectors:

Vector type
Turnaround*
shRNA vector
14-21 days
gRNA vector (single RNA)
14-21 days
gRNA vector (dual RNA)
21-27 days
Expression vector
16-22 days

Turnaround time is defined as the time from when an order is accepted to when it is shipped. It does not include waiting time for any materials that the customer needs to supply (e.g. template DNA). Adenoviral vectors may have longer turnaround time.


Deliverables

By default, we ship vectors in the format of E. coli stock. Upon request, small amount of plasmid DNA can be provided if available. Two scales of high-quality plasmid DNA can be purchased when adding vector construction service into shopping cart: Miniprep (> 10 ug) and Maxiprep (> 300 ug, endotoxinfree, sterile).

Quality control

All constructed vectors have 100%sequence guarantee. Our vectors undergo stringent quality control to ensure that they contain the correct sequence exactly as designed. Common QC includes DNA quantification, A260/280 measurement, restriction enzyme digestion, Sanger sequencing, recovering bacteria from glycerol stock, and re-transformation.

Turnaround time

We offer top-quality vector construction services at unbeatable price with rapid turnaround time. Outsourcing cloning to us is often cheaper than the cost of reagents you will need to do it yourself, let alone savings on your time, effort and headaches.