Biological Activity

27-Hydroxycholesterol is a selective estrogen receptor modulator, and it is also an agonist of the liver X receptor. 27-Hydroxycholesterol significantly increases cell proliferation of LNCaP and PC3 cells and this effect can be attenuated by estrogen receptor inhibitors. 27-Hydroxycholesterol is an oxysterol produced from cholesterol by the monooxygenase CYP27A1, which regulates intracellular cholesterol homeostasis. 27-Hydroxycholesterol also acts as an endogenous selective estrogen receptor modulator capable of increasing breast cancer growth and metastasis.

Product Citations

• 

  Exp Cell Res. 2024 Mar 8;437(1):113979.

  High level 27-HC impairs trophoblast cell invasion and migration via LXR in pre-eclampsia
  27-Hydroxycholesterol purchased from AbMole

Chemical Information

Molecular Weight	402.65
Formula	C27H46O2
CAS Number	20380-11-4
Solubility (25°C)	Ethanol 10 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Shaneabbas Raza, et al. Cancer Cell Int. The cholesterol metabolite 27-hydroxycholesterol stimulates cell proliferation via ERβ in prostate cancer cells

[2] Siker Kimbung, et al. Endocr Relat Cancer. Impact of 27-hydroxylase (CYP27A1) and 27-hydroxycholesterol in breast cancer

[3] Carolyn D DuSell, et al. Mol Endocrinol. 27-hydroxycholesterol is an endogenous selective estrogen receptor modulator

[4] X Fu, et al. J Biol Chem. 27-hydroxycholesterol is an endogenous ligand for liver X receptor in cholesterol-loaded cells