ASM10107-A655-100 | AHA1 Antibody Clinisciences

Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9 (ASM10107). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (ASM10107) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.
Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9 (ASM10107). Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (ASM10107) at 1:1000.
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9 (ASM10107). Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (ASM10107) at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).
Western Blot analysis of Human Cell lysates showing detection of Aha1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9 (ASM10107). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (ASM10107) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC/IF, IP, E
Primary Accession	Q8BK64
Other Accession	NP_666148.1
Host	Rat
Isotype	IgG2a Kappa
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Rat Anti-Mouse AHA1 Monoclonal IgG2a Kappa
Target/Specificity	Detects ~38kDa. Can run up to 45kDa on SDS Page.
Other Names	Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody
Clone Names	25F2.D9
Immunogen	Mouse Aha1
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	0.02M potassium phosphate, 0.15M sodium chloride, pH7.2
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-172 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasm \| Endoplasmic Reticulum

AHA1 Antibody