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> Ankyrin B Antibody

Ankyrin B Antibody

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Referencia ASM10234-A655-100

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Marca : Abcepta

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  • ICC/IF - Ankyrin B Antibody ASM10234
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin B Monoclonal Antibody, Clone S105-13 (ASM10234). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody (ASM10234) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Ankyrin B Antibody (D) Composite.
  • WB - Ankyrin B Antibody ASM10234
    Western Blot analysis of Rat brain membrane lysate showing detection of Ankyrin B protein using Mouse Anti-Ankyrin B Monoclonal Antibody, Clone S105-13 (ASM10234). Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody (ASM10234) at 1:1000.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC
Primary Accession Q01484
Other Accession NP_001120965.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human Ankyrin B Monoclonal IgG1
Target/Specificity Detects ~200kDa. No cross reactivity against Ankyrin-G.
Other Names ANK-2 Antibody, ANK2 Antibody, Ankyrin 2 Antibody, Ankyrin-2 Antibody, Ankyrin nonerythroid Antibody, Brain ankyrin Antibody, non-erythroid ankyrin Antibody, Ankyrin-B Antibody
Clone Names S105-13
Immunogen Synthetic peptide amino acids 203-496 of human Ankyrin-B
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-400 was sufficient for detection of Ankyrin-B in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Cytoskeleton | Myofibril | Sarcomere | M line | Apical Cell Membrane | Cell Membrane