Anti-Dnmt3a Antibody (1X206)

Application

FCM

ICC/IF

IHC

WB

Recommended DoseAntibody TypeHost SpeciesRabbitConstructionRecombinant AntibodyPurificationProA affinity purifiedAppearanceLiquidFormulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.Research BackgroundMethylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA. Related Conjugates and Formulations

Conjucates

Unconjugated

Antigen Details

Immunogen	Recombinant Protein
Uniprot ID	Q9Y6K1

Chemical Properties

Molecular Weight

Theoretical: 120 kDa.

Stability & Storage

Stability & Storage	Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
Transport	Shipping with blue ice.