Anti-GRK5 Antibody
Referencia MBS821621-0,1mL
embalaje : 0.1mL
Marca : MyBiosource
Western blot analysis of GRK5 expression in Hela (A), H446 (B), mouse kidney (C), mouse lung (D), rat kidney (E) whole cell lysates.
Immunohistochemical analysis of GRK5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
GRK5 Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821621MBS821621 is an antibody targeting GRK5. Reactive in Human, Mouse, Rat, Bovine. Host: Rabbit. Rabbit polyclonal antibody to GRK5 More details
Product Overview
Online Datasheet in PDFProduct Name
GRK5, Polyclonal Antibody
Full Product Name
Anti-GRK5 Antibody
Product Gene Name
anti-GRK5 antibody
Product Synonym Names
GPRK5; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of GRK5 protein.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
GRK5 peptide (MBS824118) is used for blocking the activity of GRK5 antibody (MBS821621)
Applications Tested/Suitable for anti-GRK5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-GRK5 antibody
WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100)
Immunohistochemistry (IHC) of anti-GRK5 antibody
Immunohistochemical analysis of GRK5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Other Notes
Small volumes of anti-GRK5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for GRK5. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
G protein-coupled receptor kinase 5
NCBI Official Synonym Full Names
G protein-coupled receptor kinase 5
NCBI Official Symbol
GRK5
NCBI Official Synonym Symbols
GPRK5
UniProt Protein Name
G protein-coupled receptor kinase 5
UniProt Synonym Protein Names
G protein-coupled receptor kinase GRK5
Protein Family
G protein-coupled receptor kinase
UniProt Gene Name
GRK5
UniProt Synonym Gene Names
GPRK5
UniProt Entry Name
GRK5_HUMAN
NCBI Protein Information
G protein-coupled receptor kinase 5; FP2025; g protein-coupled receptor kinase GRK5
NCBI Summary for GRK5
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]
Show more UniProt Comments for GRK5
GRK5: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2- mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G- protein-coupled receptor, LRP6 during Wnt signaling (in vitro). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.16; Protein kinase, Ser/Thr (non-receptor); AGC group; GRK family; GRK subfamily
Chromosomal Location of Human Ortholog: 10q26.11
Cellular Component: nucleoplasm; nuclear membrane; focal adhesion; cytoplasm; plasma membrane; nucleus
Molecular Function: G-protein coupled receptor kinase activity; protein serine/threonine kinase activity; protein binding; protein kinase C binding; phospholipid binding; ATP binding
Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; tachykinin signaling pathway; Wnt receptor signaling pathway; apoptosis; protein amino acid autophosphorylation; regulation of G-protein coupled receptor protein signaling pathway; negative regulation of apoptosis
Show more Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.16; Protein kinase, Ser/Thr (non-receptor); AGC group; GRK family; GRK subfamily
Chromosomal Location of Human Ortholog: 10q26.11
Cellular Component: nucleoplasm; nuclear membrane; focal adhesion; cytoplasm; plasma membrane; nucleus
Molecular Function: G-protein coupled receptor kinase activity; protein serine/threonine kinase activity; protein binding; protein kinase C binding; phospholipid binding; ATP binding
Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; tachykinin signaling pathway; Wnt receptor signaling pathway; apoptosis; protein amino acid autophosphorylation; regulation of G-protein coupled receptor protein signaling pathway; negative regulation of apoptosis
Research Articles on GRK5
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-GRK5 antibody
| Reference Product | PubMed Publications |
|---|---|
| ADRB2 antibody | >40 publications with GRK5 and ADRB2 |
| AVPR1A antibody | >4 publications with GRK5 and AVPR1A |
| TACR1 antibody | >2 publications with GRK5 and TACR1 |
| RHO antibody | >2 publications with GRK5 and RHO |
| SNCA antibody | >1 publications with GRK5 and SNCA |
| AGTR1 antibody | >1 publications with GRK5 and AGTR1 |
Pathways associated with anti-GRK5 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Alpha-synuclein Signaling Pathway antibodies | Alpha-synuclein Signaling Pathway Diagram |
| Calcium Regulation In The Cardiac Cell Pathway antibodies | Calcium Regulation In The Cardiac Cell Pathway Diagram |
| Chemokine Signaling Pathway antibodies | Chemokine Signaling Pathway Diagram |
| Chemokine Signaling Pathway antibodies | Chemokine Signaling Pathway Diagram |
| Endocytosis Pathway antibodies | Endocytosis Pathway Diagram |
| Endocytosis Pathway antibodies | Endocytosis Pathway Diagram |
| G Alpha (q) Signalling Events Pathway antibodies | G Alpha (q) Signalling Events Pathway Diagram |
| GPCR Downstream Signaling Pathway antibodies | GPCR Downstream Signaling Pathway Diagram |
| Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies | Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram |
| Morphine Addiction Pathway antibodies | Morphine Addiction Pathway Diagram |
Diseases associated with anti-GRK5 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Cardiovascular Diseases Antibodies | >47 publications with GRK5 and Cardiovascular Diseases |
| Heart Diseases Antibodies | >42 publications with GRK5 and Heart Diseases |
| Nervous System Diseases Antibodies | >19 publications with GRK5 and Nervous System Diseases |
| Disease Models, Animal Antibodies | >19 publications with GRK5 and Disease Models, Animal |
| Hypertension Antibodies | >18 publications with GRK5 and Hypertension |
| Hypertrophy Antibodies | >17 publications with GRK5 and Hypertrophy |
| Inflammation Antibodies | >16 publications with GRK5 and Inflammation |
| Necrosis Antibodies | >6 publications with GRK5 and Necrosis |
| Fibrosis Antibodies | >3 publications with GRK5 and Fibrosis |
| Liver Diseases Antibodies | >3 publications with GRK5 and Liver Diseases |
Organs/Tissues associated with anti-GRK5 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Heart Antibodies | >75 publications with GRK5 and Heart |
| Muscle Antibodies | >60 publications with GRK5 and Muscle |
| Brain Antibodies | >46 publications with GRK5 and Brain |
| Blood Antibodies | >36 publications with GRK5 and Blood |
| Kidney Antibodies | >27 publications with GRK5 and Kidney |
| Vascular Antibodies | >18 publications with GRK5 and Vascular |
| Eye Antibodies | >15 publications with GRK5 and Eye |
| Lung Antibodies | >10 publications with GRK5 and Lung |
| Embryonic Tissue Antibodies | >7 publications with GRK5 and Embryonic Tissue |
| Uterus Antibodies | >5 publications with GRK5 and Uterus |