anti-human CD269 (BCMA)-R-PE
Referencia MBS666371-120
embalaje : 120Tests
Marca : MyBiosource
CD269 Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666371Information: Human CD269 (BCMA, B cell maturation antigen) is predominantly expressed by mature B cells and is a type I TNF superfamily receptor member (TNFRSF17). It is highly specific for its ligand CD257 (BAFF, Blys). Antibody ANC3B1 binds to recombinant… More details
Product Overview
Online Datasheet in PDFProduct Name
CD269, Monoclonal Antibody
Full Product Name
anti-human CD269 (BCMA)-R-PE
Product Gene Name
anti-CD269 antibody
Product Synonym Names
Monoclonal anti-human CD269(BCMA)/R-PE
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
ANC3B1
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative)
Concentration
0.2 mL (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Performance
Five x 105 cultured Raji cells were washed and pre incubated ~5 minutes with 20ul of 250ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD2693/R-PE at a dilution factor of 1:50 (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.51 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD269 antibody.
Conjugation
PE
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A565/A280 ratio of 2.87.
Immunogen
Recombinant human CD269
Working Cone
10ug/mL
QC Test Method
FC binding of Raji cells :
Specification: >0.3 log shift
Result: 0.32 log shift
OD565/280 ratio:
Specification: 2.6-3.8
Result: 2.87
Specification: >0.3 log shift
Result: 0.32 log shift
OD565/280 ratio:
Specification: 2.6-3.8
Result: 2.87
Applications Tested/Suitable for anti-CD269 antibody
Flow Cytometry (FC), ELISA (EIA)
Application Notes for anti-CD269 antibody
1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
Product References and Citations for anti-CD269 antibody
1) Schneider P., J. Tschopp, et al. J. Exp. Med. 1999, 189(11):1747-1756
Other Notes
Small volumes of anti-CD269 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CD269 purchase
MBS666371 is an antibody targeting CD269. Also known as anti-human CD269 (BCMA)-R-PE. Reactive in Human. Host: Mouse.
Additional Details
NCBI/Uniprot data below describe general gene information for CD269. It may not necessarily be applicable to this product.
Protein Family
Tumor necrosis factor receptor superfamily
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.