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> Anti-HUMAN IgG IgA IgM (H&L) (GOAT) Antibody

Anti-HUMAN IgG IgA IgM (H&L) (GOAT) Antibody

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Referencia 609-101-130

embalaje : 2mg

Marca : Rockland Immunochemicals

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Teléfono : +1 850 650 7790

Background

Anti-Human IgG IgA IgM (H&L) Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Expand Background

Specifications for Human IgG IgA IgM (H&L) Antibody

Product Details

Goat Anti-Human IgG IgA IgM (H&L) Antibody - 609-101-130
Goat anti Human IgG IgA IgM Antibody, Goat Anti-Human IgG IgA IgM Antibody, Goat Anti-Human IgGAM
Goat
IgG IgA IgM
Polyclonal
IgG

Target Details

Human
Human IgG, IgA and IgM whole molecules
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgA and Human IgM.  This reagent is suitable for the detection of all Human immunoglobulin subclasses and chain combinations.

Application Details

Dot Blot, ELISA
Anti-Human IgG IgA IgM antibody has been tested by ELISA and dot blot and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Formulation

2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Expiration date is one (1) year from date of receipt.

References (2)

(). B-Cell Responses in Hospitalized Severe Acute Respiratory Syndrome Coronavirus 2–Infected Children With and Without Multisystem Inflammatory Syndrome. J Infect Dis.
Applications
EIA, ELISA
PubMed
(). Single-cell analysis suggests that ongoing affinity maturation drives the emergence of pemphigus vulgaris autoimmune disease. Cell Rep.
Applications
EIA, ELISA; ELISPOT Assay
PubMed

Related Protocols

Adherent Cell Lysis Protocol
ELISA Protocol
General Immunoblotting Protocol
Sandwich ELISA Protocol for Collagen
Suspension Cultured Cell Lysis Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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