Anti-Human IL-16
Referencia I-158-100
embalaje : 100µg
Marca : Leinco Technologies
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Teléfono : +1 850 650 7790
AntiHuman IL16
AntiHuman IL16
Product No.: I158
Target IL16 Product Type Polyclonal Antibody Alternate Names Interleukin16, FLJ16806, HsT19289, Lymphocyte Chemoattractant Factor (LCF), PrIL16 Applications CyTOF® , FC , ICC , IHC FF , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human IL16 (Accession # Q14005) Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at 20°C to 70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830552 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL16. A suggested method would be to stain cells expressing IL16 with 0.25 µg per 106 cells in a total staining volume of 100 µl followed by an appropriate secondary antibody. Western Blotting: To detect Human IL16 this polyclonal antibody can be used at a concentration of 1 µg/ml. A suitable Western blotting control is PN:T261. Additional Applications Reported In Literature ? Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 515 µg/ml. CyTOFready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Immunocytochemistry: Suitable for use at concentration of 515 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat AntiHuman Interleukin16 (IL16) recognizes Human IL16. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Interleukin 16 (lymphocyte chemoattractant factor), also known as IL16, is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. IL16 is released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. IL16 has a critical role in protecting antigen presenting cells (APC) against HIV1 infection and is used in the treatment of HIV disease.1 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Bahr, GM. et al. (1999) J. Virol. 73: 7008 |