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Anti-Nuclear Pore Complex Antibody

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Referencia 1515-NPC

embalaje : 100uL

Marca : Phosphosolutions

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Anti-Nuclear Pore Complex Antibody (39C7)

Our Anti-Nuclear Pore Complex primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, chicken, drosophila, human, mouse, and rat Nuclear pore Complex and is concentrated tissue culture supernatant. It is great for use in ICC.

Immunostaining of HeLa cells stained with anti-nuclear pore complex antibody (Cat. 1515-NPC, green, 1:100) and rabbit anti-vimentin antibody (Cat. 2105-VIM, red, 1:500).

Immunolabeling of HeLa cells stained with anti-nuclear pore complex antibody (Cat. 1515-NPC, red, 1:100) and rabbit anti-vimentin antibody (Cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA. The anti-nuclear pore complex antibody reveals strong granular labeling of the nuclei corresponding to the protein. The anti-vimentin antibody specifically labels intermediate filaments.

Immunostaining of HeLa cells stained with anti-nuclear pore complex antibody (Cat. 1515-NPC, green, 1:100) and rabbit anti-vimentin antibody (Cat. 2105-VIM, red, 1:500).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1515-NPC

Ships: 1-2 business days

Product Details

Target

Nuclear Pore Complex

Scientific Background

The nuclear pore complex (NPC) is a very large structure made up of at least 50 different proteins that span the double membrane of the nuclear envelope functioning as a gateway for macromolecular traffic between the cytoplasm and the nucleus (Nakielny and Dreyfuss,1999). Discrete nuclear pore complex proteins or nucleoporins such as NUP98, NUP180 and p62 have been implicated in autoimmune disease and cancer. Patients with primary biliary cirrhosis (PBC) frequently produce autoantibodies against p62 and NUP180 (Wilken et al., 1993; Nesher et al., 2001) while NUP98 translocations have been found in patients with acute myelogenous leukemia (AML) (Jaju et al. 2001)

Format

Concentrated tissue culture supernatant

Clonality

Monoclonal

Clone

39C7

Isotype

IgG1

Applications

ICC

Host Species

Mouse

Gene Name

Nuclear stain of multiple gene products including Nup62, Nup133

Immunogen

Yeast nuclear preparations

Immunogen Species

Yeast

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492188

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Concentrated tissue culture supernatant.

Buffer

Concentrated tissue culture supernatent + 10 mM NaN3.

Dilution Ranges

ICC: 1:50-1:100 (mammalian cells) 1:100-1:500 (yeast cells)

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the nuclear pore complex proteins.

Quality Control

Immunofluorescence performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

107 kDa nucleoporin antibody, NPC proteins antibody, NU107_HUMAN antibody, Nuclear pore complex protein Nup107 antibody, Nucleoporin 107kDa antibody, Nucleoporin Nup107 antibody, NUP107 antibody, NUP153 antibody, NUP155 antibody, NUP84 antibody, NUP98 antibody

Shipping

Blue Ice

Nuclear Pore Complex ICC Protocol: Download

General References

Nakielny S, Dreyfuss G (1999) Transport of proteins and RNAs in and out of the nucleus. Cell 99(7):677-690. PMID: 10619422
Wilken N, Kossner U, Senecal JL, Scheer U, Dabauvalle MC (1993) Nup180, a novel nuclear pore complex protein localizing to the cytoplasmic ring and associated fibrils. Journal of Cell Biology 123(6 pt 1):1345-54. PMID: 8253835
Nesher G, Margalit R, Ashkenazi YJ (2001) Anti-nuclear envelope antibodies: Clinical associations. Seminars in Arthritis and Rheumatism 30(5):313-20. PMID: 11303304
Jaju RJ, Fidler C, Haas OA, Strickson AJ, Watkins F, Clark K, Cross NC, Cheng JF, Aplan PD, Kearney L, Boultwood J, Wainscoat JS (2001) A novel gene, NSD1, is fused to NUP98 in the t(5;11)(q35;p15.5) in de novo childhood acute myeloid leukemia. Blood 98(4):1264-7. PMID: 11493482