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> Anti-SARS-CoV-2-3CLpro antibody

Anti-SARS-CoV-2-3CLpro antibody

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Referencia NB-22-72428-200

embalaje : 200μL

Marca : Neo Biotech

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Teléfono : +1 850 650 7790

  • Western blot analysis of extracts of 293T cells, using SARS-CoV-2 3CLpro antibody at 1:5000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 3CLpro fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 3CLpro rabbit polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

General Info

Host: Rabbit
Applications: WB/IF
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2-3CLpro (1-306) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:6000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Immunogen Region: 1-306
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of coronavirus 3CLpro (YP_009725295.1).
Immunogen Sequence: MESLVPGFNEKTHVQLSLPV LQVRDVLVRGFGDSVEEVLS EARQHLKDGTCGLVEVEKGV LPQLEQPYVFIKRSDARTAP HGHVMVELVAELEGIQYGRS GETLGVLVPHVGEIPVAYRK VLLRKNGNKGAGGHSYGADL KSFDLGDELGTDPYEDFQEN WNTKHSSGVTRELMRELNGG AYTRYVDNNFCGPDGYPLEC IKDLLARAGKASCTLSEQLD FIDTKRGVYCCREHEHEIA

Description

Background This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron s branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Information sourced from Uniprot.org

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