General Information
| Short Description | Rabbit polyclonal SPARC (18-303) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:100IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen Region | 18-303 |
| Immunogen Sequence | APQQEALPDETEVVEETVAE VTEVSVGANPVQVEVGEFDD GAEETEEEVVAENPCQNHHC KHGKVCELDENNTPMCVCQD PTSCPAPIGEFEKVCSNDNK TFDSSCHFFATKCTLEGTKK GHKLHLDYIGPCKYIPPCLD SELTEFPLRMRDWLKNVLVT LYERDEDNNLLTEKQKLRVK KIHENEKRLEAGDHPVELLA RDFEKNYNMYIFPVHWQFGQ LDQHPIDGYLSHTELAPLR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1). |
Additional Info
| Function | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites.an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. |
| Protein Name | SparcBasement-Membrane Protein 40Bm-40OsteonectinOnSecreted Protein Acidic And Rich In Cysteine |
| Database Links | |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneIn Or Around The Basement Membrane |
| Alternative Antibody Names | Anti-Sparc antibodyAnti-Basement-Membrane Protein 40 antibodyAnti-Bm-40 antibodyAnti-Osteonectin antibodyAnti-On antibodyAnti-Secreted Protein Acidic And Rich In Cysteine antibodyAnti-SPARC antibodyAnti-ON antibody |
Information sourced from Uniprot.org