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> Anti-Tyrosine Hydroxylase Antibody

Anti-Tyrosine Hydroxylase Antibody

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Referencia CPA3339-30ul

embalaje : 30ul

Marca : Cohesion Biosciences


  • Western blot analysis of Tyrosine Hydroxylase expression in HEK293T (A), Hela (B), SP20 (C), PC12 (D) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 58 kD)
  • Immunohistochemical analysis of Tyrosine Hydroxylase staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of Tyrosine Hydroxylase staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-Tyrosine Hydroxylase Antibody
Cat No:CPA3339
Source:Rabbit
Reactivity:H, M, R, C, D
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to Tyrosine Hydroxylase
Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Tyrosine Hydroxylase. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of Tyrosine Hydroxylase protein.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene Symbol：TH
Alternative Names：TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH
Entrez Gene (Human)： 7054;
Entrez Gene (Mouse)： 21823;
Entrez Gene (Rat)： 25085;
SwissProt (Human)： P07101;
SwissProt (Mouse)： P24529;
SwissProt (Rat)： P04177;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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