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Anti-Wnt1 Polyclonal Antibody

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Referencia NB-64-164100-50uL

embalaje : 50uL

Marca : Neo Biotech


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Anti-Wnt1 Polyclonal Antibody

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Synonyms:

Catalog No. TMAB-14183 Copy Product Info
Anti-Wnt1 Polyclonal Antibody is a Rabbit antibody targeting Wnt1. Anti-Wnt1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-Wnt1 Polyclonal Antibody is a Rabbit antibody targeting Wnt1. Anti-Wnt1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Cow,Horse)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Wnt1 Polyclonal Antibody, Unconjugated (TMAB-14183) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Wnt1 Polyclonal Antibody, Unconjugated (TMAB-14183) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Wnt1 Polyclonal Antibody, Unconjugated (TMAB-14183) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
IHC-P
IHC-Fr
IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted, extracellular space, extracellular matrix.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Wnt1
Antigen Species
Human
Gene Name
WNT1
Gene ID
7471
Protein Name
Proto-oncogene Wnt-1
Uniprot ID
P04628
Function
Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
Chemical Properties
Molecular WeightTheoretical: 39 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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