K2211-100 assays | Ascorbic Acid Colorimetric/Fluorometric Assay Kit

Background

Ascorbic Acid is a reductone and plays important roles in many biological processes. Ascorbic Acid is present in a wide variety of biological specimens and foods. Ascorbic Acid is a potent anti-oxidant, anti-viral and anti-inflammatory agent, and acts as an immune stimulant. Measurement of ascorbic acid content in different samples is important.

The Ascorbic Acid Colorimetric/Fluorometric Assay Kit provides a sensitive, fast and convenient way for detection of ascorbic acid levels in various samples based on colorimetric and fluorometric method. In the assay, the Catalyst oxidizes ascorbic acid to generate a product that interacts with the ascorbic acid probe, generating fluorescence (Ex/Em = 535/587 nm) and color (spectrophotometry at λ = 570 nm). The kit can detect 0.01-10 nmol ascorbic acid in various samples.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. 5 minutes for the reaction to complete.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

Ascorbic Acid Assay Buffer, Ascorbic Acid Probe (lyophilized), Dimethylsulfoxide (DMSO, Anhydrous), Catalyst, Ascorbic Acid Enzyme Mix (lyophilized), Ascorbic Acid Standard (20 µmole)

Ascorbic Acid Colorimetric/Fluorometric Assay Kit

Referencia K2211-100 assays

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