Bovine Interleukin 2,IL-2 ELISA Kit
Referencia CSB-E09810b-96T
embalaje : 96T
Marca : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
interleukin 2
Associated names
IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
Abbreviation
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 98-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
Recovery
| The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 96 | 92-98 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
Interleukin 2 (IL-2) is a cytokine that plays a central role in immune system regulation and T cell activation. Originally discovered as a T cell growth factor, IL-2 is primarily produced by activated CD4+ T helper cells and serves as a key mediator of adaptive immune responses. This cytokine promotes T cell proliferation, differentiation, and survival, while also influencing the development and function of regulatory T cells. IL-2 signaling occurs through the IL-2 receptor complex and activates multiple downstream pathways that maintain immune homeostasis and mount effective immune responses...
Citations
- MSA Khattab, L Haiqiang, T Shaoxun,Frontiers in Veterinary Science,2025
- M Caliskan,Cytokine,2023
- J Yang,Immunity, inflammation and disease,2022
- Y Li,3 Biotech,2020
- Z.Wang,Animal Feed Science and Technology,2020
- Seid Mohammed.et al,Biological Rhythm Research ,2017
- R. Barigye.et al,Veterinary Microbiology,2016
- Jeon JY.et al,Springerplus.,2016
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Gene References into Functions
- Pretreatment of neonatal PBMC with IL-1beta, TNF-alpha or IFN-gamma promotes mitogenic response to ConA through up-regulating the production of IL-2 and the expression of the mature IL-2 receptor.
Subcellular Location
Secreted.
Protein Families
IL-2 family
Database Links
KEGG:
STRING:
UniGene: