Canine Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit
Referencia CSB-E14930c-24T
embalaje : 24T
Marca : Cusabio
| Size | 96T,5×96T,10×96T |
Product Details
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of canine PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 91-100 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 83-104 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| The recovery of canine PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-98 | ||||
| EDTA plasma (n=4) | 94 | 89-97 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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and FAQs
This Dog PRL/LTH ELISA Kit was designed for the quantitative measurement of Dog PRL/LTH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 7.5 μIU/mL-3000 μIU/mL and the sensitivity is 7.5 μIU/mL .
Customer Reviews and Q&A
Sample type: Serum
Sample species: Dog
Sample dilution: No dilution
Review: I used the CB-E14930C canine PRL enzyme-linked immunosorbent assay kit to detect canine serum prolactin. The standard curve was fitted with a 5-parameter curve with r2=1, and the curve fitting was good. The cv value of the standard curve was less than 8.16, the back-calculation accuracy was...
By Anonymous
Sample type: Serum
Sample species: Dog
Sample dilution: No dilution
Review: I used the CSB-E1493c to detect the prolactin content in Beagle serum. The standard curve was fitted with a five-parameter model, and the R² of the curve was 1. The CV values of the standard curve ranged from 0.48% to 9.44%, and the CV values of the samples were all less than 10.15%. All...
By Anonymous