CD4 siRNA (Human)
Referencia MBS828616-15nmol
embalaje : 15nmol
Marca : MyBiosource
CD4 siRNA – T-Cell Surface Glycoprotein CD4 Isoform 1 (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828616MBS828616 is an siRNA targeting CD4. Also known as CD4 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit CD4 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
CD4, siRNA
Full Product Name
CD4 siRNA (Human)
Product Gene Name
CD4 sirna
Product Synonym Names
T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD antigen CD4
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
CD4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
51,111 Da
OMIM
186940
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CD4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for CD4 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of CD4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for CD4. It may not necessarily be applicable to this product.
NCBI Official Full Name
T-cell surface glycoprotein CD4 isoform 1
NCBI Official Synonym Full Names
CD4 molecule
NCBI Official Symbol
CD4
NCBI Official Synonym Symbols
CD4mut
UniProt Protein Name
T-cell surface glycoprotein CD4
UniProt Synonym Protein Names
T-cell surface antigen T4/Leu-3; CD_antigen: CD4
Protein Family
T-cell surface glycoprotein
UniProt Gene Name
CD4
UniProt Entry Name
CD4_HUMAN
NCBI Protein Information
T-cell surface glycoprotein CD4
NCBI Summary for CD4
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
Show more UniProt Comments for CD4
CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; lipid raft; external side of plasma membrane
Molecular Function: protein binding; enzyme binding; transmembrane receptor activity; protein homodimerization activity; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding
Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; T cell selection; cytokine production; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; defense response to Gram-negative bacterium; signal transduction; enzyme linked receptor protein signaling pathway; T cell receptor signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of T cell activation; T cell differentiation
Disease: Okt4 Epitope Deficiency
Show more Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; lipid raft; external side of plasma membrane
Molecular Function: protein binding; enzyme binding; transmembrane receptor activity; protein homodimerization activity; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding
Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; T cell selection; cytokine production; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; defense response to Gram-negative bacterium; signal transduction; enzyme linked receptor protein signaling pathway; T cell receptor signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of T cell activation; T cell differentiation
Disease: Okt4 Epitope Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with CD4 sirna
| Reference Product | PubMed Publications |
|---|---|
| FOXP3 sirna | >7607 publications with CD4 and FOXP3 |
| CCR5 sirna | >3266 publications with CD4 and CCR5 |
| IL2RA sirna | >2876 publications with CD4 and IL2RA |
| CD2 sirna | >2357 publications with CD4 and CD2 |
| CD69 sirna | >1814 publications with CD4 and CD69 |
| CD38 sirna | >1194 publications with CD4 and CD38 |
| LCK sirna | >808 publications with CD4 and LCK |
| PTPRC sirna | >99 publications with CD4 and PTPRC |
| CD8A sirna | >86 publications with CD4 and CD8A |
Pathways associated with CD4 sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Adaptive Immune System Pathway antibodies | Adaptive Immune System Pathway Diagram |
| Alpha-defensins Pathway antibodies | Alpha-defensins Pathway Diagram |
| Antigen Processing And Presentation Pathway antibodies | Antigen Processing And Presentation Pathway Diagram |
| Antigen Processing And Presentation Pathway antibodies | Antigen Processing And Presentation Pathway Diagram |
| Arf1 Pathway antibodies | Arf1 Pathway Diagram |
| Binding And Entry Of HIV Virion Pathway antibodies | Binding And Entry Of HIV Virion Pathway Diagram |
| C-MYB Transcription Factor Network Pathway antibodies | C-MYB Transcription Factor Network Pathway Diagram |
| CXCR4-mediated Signaling Events Pathway antibodies | CXCR4-mediated Signaling Events Pathway Diagram |
| Cell Adhesion Molecules (CAMs) Pathway antibodies | Cell Adhesion Molecules (CAMs) Pathway Diagram |
| Cell Adhesion Molecules (CAMs) Pathway antibodies | Cell Adhesion Molecules (CAMs) Pathway Diagram |
Diseases associated with CD4 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Inflammation Antibodies | >12831 publications with CD4 and Inflammation |
| Disease Models, Animal Antibodies | >9446 publications with CD4 and Disease Models, Animal |
| Necrosis Antibodies | >7861 publications with CD4 and Necrosis |
| Lung Diseases Antibodies | >6985 publications with CD4 and Lung Diseases |
| Liver Diseases Antibodies | >4856 publications with CD4 and Liver Diseases |
| AIDS-Related Opportunistic Infections Antibodies | >4423 publications with CD4 and AIDS-Related Opportunistic Infections |
| Carcinoma Antibodies | >3542 publications with CD4 and Carcinoma |
| Cardiovascular Diseases Antibodies | >3322 publications with CD4 and Cardiovascular Diseases |
| Neoplasms, Experimental Antibodies | >3057 publications with CD4 and Neoplasms, Experimental |
| Vascular Diseases Antibodies | >2590 publications with CD4 and Vascular Diseases |
Organs/Tissues associated with CD4 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >99471 publications with CD4 and Blood |
| Spleen Antibodies | >9666 publications with CD4 and Spleen |
| Lymph Node Antibodies | >8708 publications with CD4 and Lymph Node |
| Thymus Antibodies | >7294 publications with CD4 and Thymus |
| Bone Antibodies | >7129 publications with CD4 and Bone |
| Lung Antibodies | >6820 publications with CD4 and Lung |
| Bone Marrow Antibodies | >6279 publications with CD4 and Bone Marrow |
| Liver Antibodies | >6040 publications with CD4 and Liver |
| Intestine Antibodies | >3469 publications with CD4 and Intestine |
| Brain Antibodies | >3438 publications with CD4 and Brain |