Use code ELISA25-262 for 25% off all ELISAs through November.
25% off all ELISA kits
CGRP-I ELISA Kit
CGRP-I ELISA Kit
RayBio® Human/Mouse/Rat CGRP-I EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human, Mouse, Rat |
| Sensitivity | 0.2 ng/ml |
| Detection Range | 0.1-1,000 ng/ml |
| Specificity | This EIA kit is designed to detect human, mouse, and rat CGRP-I. |
| Compatible Sample Types | Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | Sample extraction |
| Accession Number | P06881 |
| Gene Id | 796 |
| Gene Symbols | CALCA, CALC1 |
| Protein Name / Synonyms | Calcitonin gene-related peptide 1 (Alpha-type CGRP) (Calcitonin gene-related peptide I) (CGRP-I) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Competition-based |
| Method Of Detection | Colorimetric |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Calcitonin gene-related peptide 1 (CGRP-I) is a 37-amino acid peptide produced in both peripheral and central neurons. It induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Typical Data
Standard Curve
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 µl prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl Stop Solution to each well.
- Read plate at 450 nm immediately.
Storage/Stability
Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
citations
Capsazepine-mediated TRPV1 blockade impairs angiogenesis by attenuating autophagy in perforator flaps
- Year:
- 2026
- Journal Name:
- Biochemical Pharmacology
- PubMed ID:
- 42562335
Targeting pain and inflammation: A comparative study of photobiomodulation with 532 and 660 nm lasers in rats
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (right hind paw tissue)
- Year:
- 2026
- Journal Name:
- Photochemistry and photobiology
- PubMed ID:
- 40691863
Filtered and unfiltered lipoaspirates reveal novel molecular insights and therapeutic potential for osteoarthritis treatment: a preclinical in vitro study
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (sSVF)
- Year:
- 2025
- Journal Name:
- Frontiers in Cell and Developmental Biology
- PubMed ID:
- 40083666
Novel Apocynin regulates TRPV1 activity in the trigeminal system and controls pain in a temporomandibular joint neurogenic model
- Species:
- Rat
- Sample Type:
- Cell Culture Supernatant (supernatants tissue not stated in passage)
- Year:
- 2024
- Journal Name:
- Research Square
Sensory Nerve Regulation via H3K27 Demethylation Revealed in Akermanite Composite Microspheres Repairing Maxillofacial Bone Defect
- Species:
- Mouse
- Sample Type:
- Serum (maxillofacial bone defect model)
- Year:
- 2024
- Journal Name:
- Advanced science (Weinheim, Baden-Württemberg, Germany)
- PubMed ID:
- 38874525
Associations between serum levels of alpha-calcitonin gene-related peptide and clinical features of neuromyelitis optica spectrum disorders
- Year:
- 2025
- Journal Name:
- Journal of Neuroimmunology
- PubMed ID:
- 40245783
Neuronal calcitonin gene-related peptide promotes prostate tumor growth in the bone microenvironment
- Species:
- Human; Mouse
- Sample Type:
- Serum
- Year:
- 2021
- Journal Name:
- Peptides
