Crhr1 (NM_007762) Mouse Untagged Clone

Referencia MC208333

embalaje : 10ug


Crhr1 (NM_007762) Mouse Untagged Clone

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SKU
MC208333
Crhr1 (untagged) - Mouse corticotropin releasing hormone receptor 1 (Crhr1), (10ug)
 
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Crhr1
Synonyms CRF; CRF-R; CRF1R; CRFR1; Crhr
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC208333 representing NM_007762
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACAGCGCCCGCAGCTCCGGCTCGTGAAGGCCCTTCTCCTTCTGGGGCTGAACCCCGTCTCCACCT
CCCTCCAGGATCAGCAGTGTGAGAGCCTGTCCCTGGCCAGCAATGTCTCTGGCCTGCAGTGCAATGCCTC
CGTGGACCTCATTGGCACCTGCTGGCCCAGGAGCCCTGCAGGGCAGTTGGTGGTTCGGCCCTGCCCTGCC
TTTTTCTACGGTGTCCGCTACAACACCACAAACAATGGCTACCGGGAATGCCTGGCCAACGGCAGCTGGG
CAGCCCGTGTGAATTATTCTGAGTGCCAGGAGATTCTCAACGAAGAGAAGAAGAGCAAAGTGCACTACCA
CATTGCCGTCATCATCAACTACCTGGGCCACTGCATCTCCCTGGTGGCCCTCCTGGTGGCCTTTGTCCTC
TTCCTGCGGCTCAGGAGCATCCGGTGCCTGAGGAACATCATCCACTGGAACCTCATCTCGGCTTTCATCC
TGCGCAACGCCACGTGGTTTGTGGTCCAGCTCACCGTGAGCCCCGAGGTCCACCAGAGCAACGTGGCCTG
GTGCAGGCTGGTGACAGCCGCCTACAACTACTTCCACGTAACCAACTTCTTCTGGATGTTCGGTGAGGGC
TGCTACCTGCACACAGCCATCGTACTCACGTACTCCACCGACCGTCTGCGCAAGTGGATGTTCGTCTGCA
TCGGCTGGGGTGTGCCTTTCCCCATCATTGTGGCTTGGGCCATTGGGAAACTTTACTACGACAATGAAAA
GTGCTGGTTTGGCAAACGTCCTGGAGTATATACTGACTACATCTACCAGGGCCCCATGATCCTGGTCCTG
CTGATCAACTTTATCTTTCTCTTCAACATTGTCCGCATCCTCATGACCAAACTCCGAGCATCCACCACAT
CTGAGACTATTCAGTACAGGAAGGCTGTGAAGGCCACTCTGGTGCTCTTGCCCCTCCTGGGCATCACCTA
CATGTTGTTCTTCGTCAACCCTGGGGAGGACGAGGTCTCCAGGGTTGTCTTCATCTACTTCAACTCTTTC
CTGGAGTCCTTTCAGGGCTTCTTCGTGTCTGTGTTCTATTGTTTTCTGAACAGTGAGGTCCGCTCTGCCA
TCCGGAAGAGGTGGCGGCGATGGCAGGACAAGCACTCCATCAGAGCCCGAGTGGCCCGCGCCATGTCCAT
CCCCACCTCCCCCACCAGAGTCAGCTTCCACAGCATCAAGCAGTCCACAGCAGTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_007762
Insert Size 1248 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007762.5, NP_031788.1
RefSeq Size 2428 bp
RefSeq ORF 1248 bp
Locus ID 12921
UniProt ID P35347
Cytogenetics 11 67.77 cM
Summary The protein encoded by this gene is a G-protein coupled receptor that binds corticotropin-releasing factor (CRH) and urocortin (UCN) with high affinity. The encoded protein upregulates adenylate cyclase activity and intracellular cAMP levels. This protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response, and obesity. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2015
Transcript Variant: This variant (1) encodes the longest isoform (1).
SKU Description Size
MG222960 Crhr1 (tGFP-tagged) - Mouse corticotropin releasing hormone receptor 1 (Crhr1), (10ug) 10 ug
MR222960 Crhr1 (Myc-DDK-tagged) - Mouse corticotropin releasing hormone receptor 1 (Crhr1) 10 ug
MR222960L3 Lenti ORF clone of Crhr1 (Myc-DDK-tagged) - Mouse corticotropin releasing hormone receptor 1 (Crhr1) 10 ug
MR222960L4 Lenti ORF clone of Crhr1 (mGFP-tagged) - Mouse corticotropin releasing hormone receptor 1 (Crhr1) 10 ug

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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